Structure of PDB 8cfi Chain A

Receptor sequence

>8cfiA (length=462) Species: 208964 (Pseudomonas aeruginosa PAO1)

AGFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKIL
GCIHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFA
WKGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLER
IHGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHSL
NDAIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPICA
MQACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCDAN
MLKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGF
DAHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYA
DLPAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGVS
VEGPFKPDTYRY

3D structure

• PDB: 8cfi Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry D07
• Chain: A
• Resolution: 2.02 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	T165 T166 T167 N199 I227 G230 D231 V232 E251 V252 T298 N300 V303 I321 H323 N375 H382	T158 T159 T160 N192 I220 G223 D224 V225 E244 V245 T291 N293 V296 I314 H316 N368 H375
BS02	ADE	A	H61 T63 Q65 H382 M387	H54 T56 Q58 H375 M380
BS03	SYJ	A	A37 G40 L41 K44 L396 E430 V431 E434	A30 G33 L34 K37 L389 E423 V424 E427
BS04	NAD	A	K463 Y467	K456 Y460

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8cfi, PDBe:8cfi, PDBj:8cfi
• PDBsum: 8cfi
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)