Structure of PDB 8cff Chain A

Receptor sequence

>8cffA (length=823) Species: 511 (Alcaligenes faecalis)

PNDRITLPPANAQRTNMTCHFCIVGCGYHVYKWPELQEGGRAPEQNALGL
DFRKQLPPLAVTLTPAMTNVVTEHNGRRYNIMVVPDKACVVNSGLSSTRG
GKMASYMYTPTGDGKQRLKAPRLYAADQWVDTTWDHAMALYAGLIKKTLD
KDGPQGVFFSCFDHGGAGGGFENTWGTGKLMFSAIQTPMVRIHNRPAYNS
ECHATREMGIGELNNAYEDAQLADVIWSIGNNPYESQTNYFLNHWLPNLQ
GATTSKKKERFPNENFPQARIIFVDPRETPSVAIARHVAGNDRVLHLAIE
PGTDTALFNGLFTYVVEQGWIDKPFIEAHTKGFDDAVKTNRLSLDECSNI
TGVPVDMLKRAAEWSYKPKASGQAPRTMHAYEKGIIWGNDNYVIQSALLD
LVIATHNVGRRGTGCVRMGGHQEGYTRPPYPGDKKIYIDQELIKGKGRIM
TWWGCNNFQTSNNAQALREAILQRSAIVKQAMQKARGATTEEMVDVIYEA
TQNGGLFVTSINLYPTKLAEAAHLMLPAAHPGEMNLTSMNGERRIRLSEK
FMDPPGTAMADCLIAARIANALRDMYQKDGKAEMAAQFEGFDWKTEEDAF
NDGFRRAGQPGAPAIDSQGGSTGHLVTYDRLRKSGNNGVQLPVVSWDESK
GLVGTEMLYTEGKFDTDDGKAHFKPAPWNGLPATVQQQKDKYRFWLNNGR
NNEVWQTAYHDQYNSLMQERYPMAYIEMNPDDCKQLDVTGGDIVEVYNDF
GSTFAMVYPVAEIKRGQTFMLFGYVNGIQGDVTTDWTDRNIIPYYKGTWG
DIRKVGSMEEFKRTVSFKSRRFA

3D structure

• PDB: 8cff Arsenite oxidase in complex with antimonite and arsenite oxyanions: Insights into the catalytic mechanism.
• Chain: A
• Resolution: 1.57 Å

Enzymatic activity

• Enzyme Commision number: 1.20.9.1: arsenate reductase (azurin).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MGD	A	G170 H195 N196 W389 H423 G456 C457 N458 T462 I513 N514 L515 A530 D563 N700 R702 Q708 T709 Y711 F774 Y797	G168 H193 N194 W387 H421 G454 C455 N456 T460 I511 N512 L513 A528 D561 N698 R700 Q706 T707 Y709 F772 Y795
BS02	MGD	A	R101 G232 N233 E237 S238 D277 P278 R279 D306 E384 K385 G386 G422 H423 W697 N699 G701 R702 N703 N704 V706 W707 Q708 K798	R99 G230 N231 E235 S236 D275 P276 R277 D304 E382 K383 G384 G420 H421 W695 N697 G699 R700 N701 N702 V704 W705 Q706 K796
BS03	F3S	A	C21 C24 G27 C28 Y30 S99 R101 G102 T240 N241	C19 C22 G25 C26 Y28 S97 R99 G100 T238 N239
BS04	AST	A	H195 N196 E203 R419 H423	H193 N194 E201 R417 H421

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding
• GO:0050611 arsenate reductase (azurin) activity
• GO:0051536 iron-sulfur cluster binding
• GO:0051538 3 iron, 4 sulfur cluster binding

Biological Process

• GO:0045333 cellular respiration

Cellular Component

• GO:1990204 oxidoreductase complex

External links

• PDB: RCSB:8cff, PDBe:8cff, PDBj:8cff
• PDBsum: 8cff
• PubMed: 37442232
• UniProt: Q7SIF4|AIOA_ALCFA Arsenite oxidase subunit AioA (Gene Name=aioA)