Structure of PDB 8c8w Chain A

Receptor sequence
>8c8wA (length=1032) Species: 1404 (Priestia megaterium) [Search protein sequence]
MKEVNVRESSQEREQRIQQQWQKGNVFQQSVQNREGYPSFVFYEGPPTAN
GLPGVHHALGRTIKDVVARYKTMTGHQVIRKAGWDTHGLPVELGVEKQLG
ISGKHDIEAYGVEAFINKCKESVFVYEKQWRTFTEQLGYWVDMEDPYITL
ENSYIESVWNVLGTIHDKGLLYKGHRVSPYCPSCQTSLSSHEVAQGYKDV
KDLTVTVKFKVKNRDNEYFLGWTTTPWTLPSNVALAVHEEMSYVRAEQGD
SVYIVAEALADKVLKGEYSVLSHHKGNELKGMSYEPPFNFVKVEKGHEVV
TADYVTDQSGTGVVHLAPAYGEDDYRVVKENGFSFVNVVDEKGQYTSEVP
PFQGRFVKDCDVDIVRYLANQDVLYHKEKHEHSYPFCWRCDSPLLYYANE
SWFIQTTALKEQFLKNNESVKWYPDHIKHGRFGKFLENMVDWNISRKRYW
GTPLNVWECEGCQHQVAPKSIKELQKHASHYVDDSIELHKPYVDDVQLTC
PVCSGEMKRTPEVIDVWFDSGSMPFAQYHYPFENSELFQKQFPADVIAEG
IDQTRGWFYSLMAVSTLFTGKAPYKRVLSLGHVLDENGQKMSKSKGNALD
PVDLIHTFGADALRWALLADSAPWNPKKFSERVVQEAKSKVIDTLVNVYG
FYVLYAKLDGYDPEQTYELKKTKLDEWILSRLHSTVKRATVHLEDYGFTS
AAREIAVFIEELSNWYVRRSRDRFWSEGMDGEKAAAYDTLHEVLVTLSQL
LAPFTPFVADDVHENLTGKSVHLADYPACDQTKVNEKLEKEMAAVLQVVE
LGRSIRNTHSLKVKQPLQSLSLVVTEEDVEWKAYRDVIKDELNVKNFNVE
QDDDKLVSYVLKLDFKQAGPKFGKQVNEVNQALKNLSEEKGKEFVEQGKL
SVTLASGENLTLETEDVLVEKVPKEGFAVASNGMYTAVLDTALTEELVQE
GVAREVIRAVQDYRKKLDLPVNSRINLELSGDEEVQKAVAKFETLLQENL
LLHSLSVKETIKNGETVKVGTKQVVLRVLNQS
3D structure
PDB8c8w Antibiotic hyper-resistance in a class I aminoacyl-tRNA synthetase with altered active site signature motif
ChainA
Resolution2.29 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.5: isoleucine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ILA A P46 P47 T48 H56 D85 W517 S520 E549 G550 D552 Q553 W557 H582 V583 P46 P47 T48 H56 D85 W517 S520 E549 G550 D552 Q553 W557 H582 V583
BS02 ZN A C459 C462 C500 C503 C459 C462 C500 C503
BS03 ZN A C181 C184 C387 C390 C181 C184 C387 C390
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004822 isoleucine-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006428 isoleucyl-tRNA aminoacylation
GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8c8w, PDBe:8c8w, PDBj:8c8w
PDBsum8c8w
PubMed37679387
UniProtA0A0B6AVD3

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