Structure of PDB 8c8v Chain A

Receptor sequence
>8c8vA (length=1032) Species: 1348623 (Priestia megaterium NBRC 15308 = ATCC 14581) [Search protein sequence]
MKEVNVRESSQEREQRIQQQWQKGNVFQQSVQNREGYPSFVFYEGPPTAN
GLPHVGHALGRTIKDVVARYKTMTGHQVIRKAGWDTHGLPVELGVEKQLG
ISGKHDIEKYGVEAFINKCKESVFVYEKQWRTFTEQLGYWVDMEDPYITL
ENSYIESVWNVLGTIHDKGLLYKGHRVSPYCPSCQTSLSSHEVAQGYKDV
KDLTVTVKFKVKNRDNEYFLGWTTTPWTLPSNVALAVHEEMSYVRAEQGD
SVYIVAEALADKVLKGEYSVLSHHKGNELKGMSYEPPFNFVKVEKGHEVV
TADYVTDQSGTGVVHLAPAYGEDDYRVVKENGFSFVNVVDEKGQYTSEVP
PFQGRFVKDCDVDIVRYLANQDVLYHKEKHEHSYPFCWRCDSPLLYYANE
SWFIQTTALKEQFLKNNESVKWYPDHIKHGRFGKFLENMVDWNISRKRYW
GTPLNVWECEGCQHQVAPKSIKELQKHASHYVDDSIELHKPYVDDVQLTC
PVCSGEMKRTPEVIDVWFDSGSMPFAQYHYPFENSELFQKQFPADVIAEG
IDQTRGWFYSLMAVSTLFTGKAPYKRVLSLGHVLDENGQKMSKSKGNALD
PVDLIHTFGADALRWALLADSAPWNPKKFSERVVQEAKSKVIDTLVNVYG
FYVLYAKLDGYDPEQTYELKKTKLDEWILSRLHSTVKRATVHLEDYGFTS
AAREIAVFIEELSNWYVRRSRDRFWSEGMDGEKAAAYDTLHEVLVTLSQL
LAPFTPFVADDVHENLTGKSVHLADYPACDQTKVNEKLEKEMAAVLQVVE
LGRSIRNTHSLKVKQPLQSLSLVVTEEDVEWKAYRDVIKDELNVKNFNVE
QDDDKLVSYVLKLDFKQAGPKFGKQVNEVNQALKNLSEEKGKEFVEQGKL
SVTLASGENLTLETEDVLVEKVPKEGFAVASNGMYTAVLDTALTEELVQE
GVAREVIRAVQDYRKKLDLPVNSRINLELSGDEEVQKAVAKFETLLQENL
LLHSLSVKETIKNGETVKVGTKQVVLRVLNQS
3D structure
PDB8c8v Antibiotic hyper-resistance in a class I aminoacyl-tRNA synthetase with altered active site signature motif
ChainA
Resolution2.2 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.5: isoleucine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A C181 C184 C387 C390 C181 C184 C387 C390
BS02 ZN A C459 C462 C500 C503 C459 C462 C500 C503
BS03 ILA A P46 P47 T48 H54 G56 H57 D85 W517 S520 E549 G550 D552 Q553 H582 V583 P46 P47 T48 H54 G56 H57 D85 W517 S520 E549 G550 D552 Q553 H582 V583
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004822 isoleucine-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006428 isoleucyl-tRNA aminoacylation
GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8c8v, PDBe:8c8v, PDBj:8c8v
PDBsum8c8v
PubMed37679387
UniProtA0A0B6AVD3

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