Structure of PDB 8c8j Chain A

Receptor sequence

>8c8jA (length=698) Species: 9606 (Homo sapiens)

KLNNLLLNDYWVHNEMKAEIKMFFETNENKDTTYQNLWDAFKAVCRGKFI
ALNANQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANKLENLEEMDTFL
DTYTLPRLNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQR
YKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTKKENFRPI
SLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVI
QHINRAKDKNHMIISIDAEKAFDKIQQPFMLKTLNKLGIDGTYFKIIRAI
YDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIRQEK
EIKGIQLGKEEVKLSLFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKI
NVQKSQAFLYTNNRQTESQIMGELPFTIASKRIKYLGIQLTRDVKDLFKE
NYKPLLKEIKEETNKWPCSWVGRINIVKMAILPKVIYRFNAIPIKLPMTF
FTELEKTTLKFIWNQTLPDFKLYYKATVTKTAWYWYQNRDIDQWNRTEPS
YNYLIFDKPEKNGKDSLFNKWCWENWLAICRKLKLDPFLTPYTKINSRWI
KDLNVKPKTIKTLEENLGITIQDIGVGKDFMSKTPKAMATKDKIDKWDLI
KLKSFCTAKETTIRVNRQPTTWEKIFATYSSDKGLISRIYNELKQIYH

3D structure

• PDB: 8c8j Long Interspersed Nuclear Element 1 (LINE-1) reverse transcriptase ternary complex with hybrid duplex and dTTP
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.49: RNA-directed DNA polymerase.
  3.1.21.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	F566 F700 D703 L769 G770 M815 P819 K820 Y823	F233 F367 D370 L436 G437 M479 P483 K484 Y487
BS02	rna	A	K469 S470 I515 I517 I533 L535 K541 K545 F566 I567 P568 M570 Q571 I642 N644 G645 G660 P662 Y878 K1047 G1048 I1050 S1051	K136 S137 I182 I184 I200 L202 K208 K212 F233 I234 P235 M237 Q238 I309 N311 G312 G327 P329 Y523 K683 G684 I686 S687
BS03	TTP	A	K519 R531 D600 A601 E602 K603 A604 F605 Q659 D702 N734 K737	K186 R198 D267 A268 E269 K270 A271 F272 Q326 D369 N401 K404
BS04	MG	A	K556 I559	K223 I226
BS05	MG	A	D600 A601 D702	D267 A268 D369

External links

• PDB: RCSB:8c8j, PDBe:8c8j, PDBj:8c8j
• PDBsum: 8c8j
• PubMed: 38096902
• UniProt: O00370|LORF2_HUMAN LINE-1 retrotransposable element ORF2 protein