Structure of PDB 8c4v Chain A

Receptor sequence

>8c4vA (length=1308) Species: 11602 (Hantaan virus 76-118)

YIPDYKGMEPIGANIEDLVDYSKDWLSRARNFSFFEVKGTAVFECFNSNE
ANHCQRYPMSRKPRNFLLIQCSLITSYKPATTLSDQIDSRRACSYILNLI
PDTPASYLIHDMAYRYINLTREDMINYYAPRIQFKQTQNVREPGTFKLTS
SMLRAESKAMLDLLNNQIESLNIASHIVQSESVSLITKILSDLELNITTV
LDKFFQNETQKYLIDVLKKTTAWHIGHLIRDITESLIAHSGLKRSKYWSL
HSYNNGNVILFILPSKSLEVAGSFIRFITVFRIGPGLVDKDNLDTILIDG
DSQWGVSKVMSIDLNRLLALNIAFEKALIATATWFQYYTEDQGQFPLQYA
IRSVFANHFLLAICQKMKLCAIFDNLRYLIPAVTSLYSGFPSLIEKLFER
PFKSSLEVYIYYNIKSLLVALAQNNGVYPSFMSRIVYKHYRSLISEVTTC
FFLFEKGLHGNMNEEAKIHLETVEWALKFREKEEKYGESLVENGYMMWEL
RANAELAEQQLYCQDAIELAAIELNKVLATKSSVVANSILSKNWEEPYFS
QTRNISLKGMSGQVQEDGHLSSSVTIIEAIRYLSNSRHNPSLLKLYEETR
EQKAMARIVRKYQRTEADRGFFITTLPTRCRLEIIEDYYDAIAKNISEEY
ISYGGEKKILAIQGALEKALRWASGESFIELSNHKFIRMKRKLMYVSADA
TKWSPGDNSAKFRRFTSMLHNGLPNNKLKNCVIDALKQVYKTDFFMSRKL
RNYIDSMESLDPHIKQFLDFFPDGHHGEVKGNWLQGNLNKCSSLFGVAMS
LLFKQVWTNLFPELDCFFEFAHHSDDALFIYGYLEPVDDGTDWFLFVSQQ
IQAGHLHWFSVNTEMWKSMFNLHEHILLLGSIKISPKKTTVSPTNAEFLS
TFFEGCAVSIPFVKILLGSLSDLPGLGYFDDLAAAQSRCVKALDLGASPQ
VAQLAVALCTSKVERLYGTAPGMVNHPAAYLQVKHTDTPIPLGGNGAMSI
MELATAGIGMSDKNLLKRALLGYSHKRQKSMLYILGLFKFLMKLSDETFQ
HERLGQFSFIGKVQWKIFTPKSEFEFADMYTSKFLELWSSQHVTYDYIIP
KGRDNLLIYLVRKLNDPSIVTAMTMQSPLQLRFRMQAKQHMKVCRLDGEW
VTFREVLAAANSFAENYSATSQDMDLFQTLTWKDFLNGIHCDVIPTKQVQ
RAKVARTFTVRESIPAVIGYKFAVTVEEMSDVLDTAKFPDSLSVDLKTMK
DGVYRELGLDISLPDVMKRIAPMLYKSRVVIVQGNVEGTAEAICRYWLKS
MSLVKTIR

3D structure

• PDB: 8c4v Structures of active Hantaan virus polymerase uncover the mechanisms of Hantaviridae genome replication.
• Chain: A
• Resolution: 3.14 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R281 Y282 L388 L389 Q401 I402 K496 K516 G522 F524 R526 K558 V559 M560 S561 R566 K616 M617 K618 P651 K653 G733 N734 N736 Y1026 I1027 M1030 S1032 L1033 D1034 H1036 I1037 F1040	R56 Y57 L163 L164 Q167 I168 K246 K266 G272 F274 R276 K308 V309 M310 S311 R316 K366 M367 K368 P401 K403 G460 N461 N463 Y753 I754 M757 S759 L760 D761 H763 I764 F767
BS02	rna	A	T374 S375 L492 K496 S517	T149 S150 L242 K246 S267
BS03	rna	A	R826 K831 G832 V882 K884 Y885 F894 F895 I896 R902 E909 Y926 G927 G928 Q1058 G1059 F1202 D1203 A1206 A1207 S1210 I1281 M1398 P1561 Y1564 K1565	R553 K558 G559 V609 K611 Y612 F621 F622 I623 R629 E636 Y653 G654 G655 Q785 G786 F929 D930 A933 A934 S937 I1008 M1125 P1272 Y1275 K1276
BS04	rna	A	F725 T825 L830 N1062 H1096 S1097 D1098 L1172 S1173 K1187 I1188 G1191 S1194 R1211 S1391 I1392 A1395 Y1564	F452 T552 L557 N789 H823 S824 D825 L899 S900 K914 I915 G918 S921 R938 S1118 I1119 A1122 Y1275
BS05	rna	A	P304 A305 T306 T307 Y353 P355 R356 Q460 I464 K468 W473 H477 R480 A488 G491 R494 L1231 S1234 R1238 A1243 P1244 G1245 M1246 F1406 A1410 H1413 M1414	P79 A80 T81 T82 Y128 P130 R131 Q210 I214 K218 W223 H227 R230 A238 G241 R244 L958 S961 R965 A970 P971 G972 M973 F1133 A1137 H1140 M1141
BS06	MG	A	A971 D1099	A698 D826

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004519 endonuclease activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039689 negative stranded viral RNA replication
• GO:0039694 viral RNA genome replication
• GO:0039696 RNA-templated viral transcription
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044220 host cell perinuclear region of cytoplasm

External links

• PDB: RCSB:8c4v, PDBe:8c4v, PDBj:8c4v
• PDBsum: 8c4v
• PubMed: 37221161
• UniProt: P23456|L_HANTV RNA-directed RNA polymerase L (Gene Name=L)