Structure of PDB 8c4u Chain A

Receptor sequence

>8c4uA (length=1260) Species: 11602 (Hantaan virus 76-118)

YIPDYKGMEPIGANIEDLVDYSKDWLSRARNFSFFEVKGTAVFECFNSNE
ANHCQRYPMSRKPRNFLLIQCSLITSYKPATTLSDQIDSRRACSYILNLI
PDTPASYLIHDMAYRYINLTREDMINYYAPRIQFKQTQNVREPGTFKLTS
SMLRAESKAMLDLLNNQIESLNIASHIVQSESVSLITKILSDLELNITTV
LDKFFQNETQKYLIDVLKKTTAWHIGHLIRDITESLIAHSGLKRSKYWSL
HSYNNGNVILFILPSKSLEVAGSFIRFITVFRIGPGLVDKDNLDTILIDG
DSQWGVSKVMSIDLNRLLALNIAFEKALIATATWFQYYTEDQGQFPLQYA
IRSVFANHFLLAICQKMKLCAIFDNLRYLIPAVTSLYSGFPSLIEKLFER
PFKSSLEVYIYYNIKSLLVALAQNNKSGVYPSFMSRIVYKHYRSLISEVT
TCFFLFEKGLHGNMNEEAKIHLETVEWALKFREKEEKYGESLVENGYMMW
ELRANAELAEQQLYCQDAIELAAIELNKVLATKSSVVANSILSKNWEEPY
FSQTRNISLKGMSGQVQEDGHLSSSVTIIEAIRYLSNSRHNPSLLKLYEE
TREQKAMARIVRKYQRTEADRGFFITTLPTRCRLEIIEDYYDAIAKNISE
EYISYGGEKKILAIQGALEKALRWASGESFIELSKFIRMKRKLMYVSADA
TKWSPGDNSAKFRRFTSMLHNGLPNNKLKNCVIDALKQVYKTDFFMSRKL
RNYIDSMESLDPHIKQFLDFFPDGHHGEVKGNWLQGNLNKCSSLFGVAMS
LLFKQVWTNLFPELDCFFEFAHHSDDALFIYGYLEPVDDGTDWFLFVSQQ
IQAGHLHWFSVNTEMWKSMFNLHEHILLLGSIKISPKKTTVSPTNAEFLS
TFFEGCAVSIPFVKILLGSLSDLPGLGYFDDLAAAQSRCVKALDLGASPQ
VAQLAVALCTSKVERLYGTAPGMVNHPAAYLQVKHTDTPIPLGGNGAMSI
MELATAGIGMSDKNLLKRALLGYSHKRQKSMLYILGLFKFLMKLGQFSFI
GVQWKIFTPKSEFEFADMYTSKFLELWSSQHVTYDYIIPKGRDNLLIYLV
RKLNDPSIVTAMTMQSPLQLRFRMQAKQHMKVCRLDGEWVTFREVLAAAN
SFAENYSATSQDMDLFQTLTSCTFSKEYAWKDFLNGIHCDVIPTKQVQRA
KVARTFTAVIGYKFAVTVEEMSDVLDTARVVIVQGNVEGTAEAICRYWLK
SMSLVKTIRV

3D structure

• PDB: 8c4u Structures of active Hantaan virus polymerase uncover the mechanisms of Hantaviridae genome replication.
• Chain: A
• Resolution: 3.36 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Y282 L388 L389 I402 K516 G522 F524 R526 K558 V559 S561 R566 K616 M617 K618 N736 A739 Y1026 M1030 S1032 F1040	Y57 L163 L164 I168 K266 G272 F274 R276 K308 V309 S311 R316 K366 M367 K368 N465 A468 Y753 M757 S759 F767
BS02	rna	A	T374 L492 K496 S517 E519 V520 K831 G832 R880 V882 Y885 F894 F895 I896 G927 G928 E929 G1059 N1062 F1341 T1342 K1344 T1397 Q1399 S1400 Q1403 R1407	T149 L242 K246 S267 E269 V270 K560 G561 R609 V611 Y614 F623 F624 I625 G656 G657 E658 G786 N789 F1057 T1058 K1060 T1113 Q1115 S1116 Q1119 R1123

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004519 endonuclease activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039689 negative stranded viral RNA replication
• GO:0039694 viral RNA genome replication
• GO:0039696 RNA-templated viral transcription
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044220 host cell perinuclear region of cytoplasm

External links

• PDB: RCSB:8c4u, PDBe:8c4u, PDBj:8c4u
• PDBsum: 8c4u
• PubMed: 37221161
• UniProt: P23456|L_HANTV RNA-directed RNA polymerase L (Gene Name=L)