Structure of PDB 8c4t Chain A

Receptor sequence

>8c4tA (length=1268) Species: 11602 (Hantaan virus 76-118)

YIPDYKGMEPIGANIEDLVDYSKDWLSRARNFSFFEVKGTAVFECFNSNE
ANHCQRYPMSRKPRNFLLIQCSLITSYKPATTLSDQIDSRRACSYILNLI
PDTPASYLIHDMAYRYINLTREDMINYYAPRIQFKQTQNVREPGTFKLTS
SMLRAESKAMLDLLNNQIESLNIASHIVQSESVSLITKILSDLELNITTV
LDKFFQNETQKYLIDVLKKTTAWHIGHLIRDITESLIAHSGLKRSKYWSL
HSYNNGNVILFILPSKSLEVAGSFIRFITVFRIGPGLVDKDNLDTILIDG
DSQWGVSKVMSIDLNRLLALNIAFEKALIATATWFQYYTEDQGQFPLQYA
IRSVFANHFLLAICQKMKLCAIFDNLRYLIPAVTSLYSGFPSLIEKLFER
PFKSSLEVYIYYNIKSLLVALAQNVYPSFMSRIVYKHYRSLISEVTTCFF
LFEKGLHGNMNEEAKIHLETVEWALKFREKEEKYGESLVENGYMMWELRA
NAELAEQQLYCQDAIELAAIELNKVLATKSSVVANSILSKNWEEPYFSQT
RNISLKGMSGQVQEDGHLSSSVTIIEAIRYLSNSRHNPSLLKLYEETREQ
KAMARIVRKYQRTEADRGFFITTLPTRCRLEIIEDYYDAIAKNISEEYIS
YGGEKKILAIQGALEKALRWASGESFIELSFIRMKRKLMYVSADATKWSP
GDNSAKFRRFTSMLHNGLPNNKLKNCVIDALKQVYKTDFFMSRKLRNYID
SMESLDPHIKQFLDFFPDGHHGEVKGNWLQGNLNKCSSLFGVAMSLLFKQ
VWTNLFPELDCFFEFAHHSDDALFIYGYLEPVDWFLFVSQQIQAGHLHWF
SVNTEMWKSMFNLHEHILLLGSIKISPKKTTVSPTNAEFLSTFFEGCAVS
IPFVKILLGSLSDLPGLGYFDDLAAAQSRCVKALDLGASPQVAQLAVALC
TSKVERLYGTAPGMVNHPAAYLQVKHTDTPIPLGGNGAMSIMELATAGIG
MSDKNLLKRALLGYSHKRQKSMLYILGLFKFLMKLSDETFQFSFIGEFEF
ADMYTSKFLELWSSQHVTYDYIIPKGRDNLLIYLVRKLNDPSIVTAMLQL
RFRMQAKQHMKVCRLDGEWVTFREVLAAANSFAENYSATSQDMDLFQTLT
SCTAWKDFLNGIHCDVIPTKQVQRAKVARTFTSIPAVIGYKFAVTVEEMS
DVLDTAKFPDSLSVDLKTMKDGVYRESLPDVMKRIARVVIVQGNVEGTAE
AICRYWLKSMSLVKTIRV

3D structure

• PDB: 8c4t Structures of active Hantaan virus polymerase uncover the mechanisms of Hantaviridae genome replication.
• Chain: A
• Resolution: 3.23 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R281 Y282 K287 R289 L389 N391 I402 G522 F524 K558 V559 S561 R566 K616 M617 K618 K653 G733 N736 A739 Y1026 I1027 M1030 S1032 D1034 I1037 F1040	R56 Y57 K62 R64 L164 N166 I168 G272 F274 K308 V309 S311 R316 K366 M367 K368 K403 G458 N461 A464 Y748 I749 M752 S754 D756 I759 F762

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004519 endonuclease activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039689 negative stranded viral RNA replication
• GO:0039694 viral RNA genome replication
• GO:0039696 RNA-templated viral transcription
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044220 host cell perinuclear region of cytoplasm

External links

• PDB: RCSB:8c4t, PDBe:8c4t, PDBj:8c4t
• PDBsum: 8c4t
• PubMed: 37221161
• UniProt: P23456|L_HANTV RNA-directed RNA polymerase L (Gene Name=L)