Structure of PDB 8c41 Chain A

Receptor sequence

>8c41A (length=720) Species: 1313 (Streptococcus pneumoniae)

KLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQAILPLRGKVINTAK
AKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDADTDGAHI
QTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGEL
EELRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARA
ERRVNVLMGDKVEPRRKWIEDNVKFTLEEATVFHMSNIQNMSLEDIMGER
FGRYSKYIIQDRALPDIRDGLKPVQRRILYSMNKDSNTFDKSYRKSAKSV
GNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMHGNNGSMDGDPPAAM
RYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFPNLLVNG
STGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTG
AIIQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKA
NLVKKIDDVRVNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLF
KYTDLQINYNFNMVAIDNFTPRQVGIVPILSSYIAHRREVILARSRFDKE
KAEKRLHIVEGLIRVISILDEVIALIRASENKADAKENLKVSYDFTEEQA
EAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLAAIIGDERTMYNLMKKE
LREVKKKFATPRLSSLEDTA

3D structure

• PDB: 8c41 The nature of the molecular interactions at high resolution of the Streptococcus pneumoniae topoisomerase IV-DNA complex with the novel fluoroquinolone Delafloxacin.
• Chain: A
• Resolution: 2.393 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E433 G457 K458 D510 R1028 K1038 H1074 H1076 S1080 R1087 K1093 I1170 E1262	E19 G43 K44 D96 R262 K272 H308 H310 S314 R321 K327 I404 E496
BS02	dna	A	K458 N461 K464 H513 V626 R629 R630 Y1118 I1170 S1171 G1173 Y1174 A1175 R1235 N1326	K44 N47 K50 H99 V212 R215 R216 Y352 I404 S405 G407 Y408 A409 R469 N560
BS03	MG	A	F1316 K1317 T1319 Q1322	F550 K551 T553 Q556
BS04	TE9	A	D435 R456 S1079	D21 R42 S313

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:8c41, PDBe:8c41, PDBj:8c41
• PDBsum: 8c41
• UniProt: P72525|PARC_STRPN DNA topoisomerase 4 subunit A (Gene Name=parC)