Structure of PDB 8btg Chain A

Receptor sequence

>8btgA (length=334) Species: 1423 (Bacillus subtilis)

NMLNPKYTFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKT
HLMHAIGHYVIDHNPSAKVVYLSSEKFTNEFINSIRDNKAVDFRNRYRNV
DVLLIDDIQFLAGKEQTQEEFFHTFNTLHEESKQIVISSDRPPKEIPTLE
DRLRSRFEWGLITDITPPDLETRIAILRKKAKAEGLDIPNEVMLYIANQI
DSNIRELEGALIRVVAYSSLINKDINADLAAEALKDSKPKVITIKEIQRV
VGQQFNIKLEDFKAKKRTKSVAFPRQIAMYLSREMTDSSLPKIGEEFGGR
DHTTVIHAHEKISKLLADDEQLQQHVKEIKEQLK

3D structure

• PDB: 8btg The bacterial replication origin BUS promotes nucleobase capture.
• Chain: A
• Resolution: 3.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R194 R412 D413 T415 T416 H419 K423	R86 R300 D301 T303 T304 H307 K311
BS02	dna	A	F189 I190 I193 R194 A198 R202 K222 E223 E228 R379 R395 S401 L402 P403 H414 H419	F81 I82 I85 R86 A90 R94 K114 E115 E120 R267 R283 S289 L290 P291 H302 H307
BS03	ATP	A	Y115 G154 L155 G156 T158 I284 I312 R313	Y7 G46 L47 G48 T50 I176 I204 R205
BS04	ATP	A	R264 W267	R156 W159

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003688 DNA replication origin binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding
• GO:0043565 sequence-specific DNA binding

Biological Process

• GO:0006260 DNA replication
• GO:0006270 DNA replication initiation
• GO:0006275 regulation of DNA replication

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:1990101 DnaA-oriC complex

External links

• PDB: RCSB:8btg, PDBe:8btg, PDBj:8btg
• PDBsum: 8btg
• PubMed: 38097584
• UniProt: P05648|DNAA_BACSU Chromosomal replication initiator protein DnaA (Gene Name=dnaA)