Structure of PDB 8bew Chain A

Receptor sequence

>8bewA (length=571) Species: 2325 (Thermoanaerobacter kivui)

MNMVMLTIDGKQVQVEKGTTIKKAAEKLGIEIPGLCDDNDLKPFGACRLC
VVEDARGNLVASCHTPVREGMVVKTNSPKVLKARRVILELLLSSHNADCF
ECDKNLHCKLQKYAYELNIRNIRFKGEKRNYEIKDNGPIYYDPNKCILCG
KCVRICEEVQHICAIDFASRGFKAYISTPFEKPLLESDCIFCGQCVRVCP
TGALAEKTDIERIYEAISDPNKVVVVQVAPAVRVALGEEFGLEPGEIVTG
KMVAALKRLGFDKVFDTQFAADMTIVEETAELVERLEKGENFPMFTSCCP
SWILAVEKFYPELIPNISTARSPQQIFGAIAKNYYAKKIGVARENMFVVS
VMPCIGKKFEATRPEFNNDVDAVLTTRELARMIKESGIDFIKLEEENFDS
PLGESTGAAAIFGVTGGVMEAALRTAYSIMTGEELEGDKIEFTAVRGLEG
IKEAEVDIKGKKVRIAIANGIGNAKKLIEKIKSGETKYDFVEVMACPGGC
MSGGGQPYTDDPEFRKKRMEGIYKNDRNLPKRKSHENEEVKKVYEEYYEK
PCGPKAHEELHTHYHSRKKEY

3D structure

• PDB: 8bew Molecular Basis of the Electron Bifurcation Mechanism in the [FeFe]-Hydrogenase Complex HydABC.
• Chain: A
• Resolution: 3.49 Å

Enzymatic activity

• Enzyme Commision number: 1.12.1.3: hydrogen dehydrogenase (NADP(+)).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	C299 S301 C354 C496 C500	C299 S301 C354 C496 C500
BS02	FES	A	C36 D37 G45 C47 C50 C63	C36 D37 G45 C47 C50 C63
BS03	SF4	A	H95 C99 C102 C108 G202	H95 C99 C102 C108 G202
BS04	SF4	A	C146 L148 C149 C152 I176 C199 P200	C146 L148 C149 C152 I176 C199 P200
BS05	SF4	A	C156 C189 C192 G193 C195	C156 C189 C192 G193 C195

Gene Ontology

Molecular Function

• GO:0005506 iron ion binding
• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0008901 ferredoxin hydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0050583 hydrogen dehydrogenase (NADP+) activity
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0042773 ATP synthesis coupled electron transport
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:8bew, PDBe:8bew, PDBj:8bew
• PDBsum: 8bew
• PubMed: 36811855
• UniProt: A0A097ATG3