Structure of PDB 8b9g Chain A

Receptor sequence

>8b9gA (length=953) Species: 7227 (Drosophila melanogaster)

EQRDMNEAEAFDVNAAIHGNWTIENAKERLNIYKQTNNIRDDYKYTPVGP
EHARSFLAELSIYVPALNRTVTARESGSNKKSASKSCALSLVRQLFHLNV
IEPFSGTLEQLKPYPVKLSPNLINKIDEVIKGLDLPVVNSSVIPWAPPQA
NWNTWHACNIDEGELATTSIDDLSMDYERSLRDRRQNDNEYRQFLEFREK
LPIAAMRSEILTAINDNPVVIIRGNTGCGKTTQIAQYILDDYICSGQGGY
ANIYVTQPRRISAISVAERVARERCEQLGDTVGYSVRFESVFPRPYGAIL
FCTVGVLLRKLEAGLRGVSHIIVDEIHERDVNSDFLLVILRDMVDTYPDL
HVILMSATIDTTKFSKYFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPS
AESINYNKVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPGA
ILVFLPGWNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEP
VPEGVTKIILSTNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATV
WASKTNLEQRKGRAGRVRPGFCFTLCSRARFQALEDNLTPEMFRTPLHEM
ALTIKLLRLGSIHHFLSKALEPPPVDAVIEAEVLLREMRCLDANDELTPL
GRLLARLPIEPRLGKMMVLGAVFGCADLMAIMASYSSTFSEVFSLDIGQR
RLANHQKALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDWKGLQMS
TMNVIWDAKQQLLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLC
LGLYPNICVHKEKRKVLTTESKAALLHKTSVNCSNLAVTFPYPFFVFGEK
IRTRAVSCKQLSMVSPLQVILFGSRKIDLAANNIVRVDNWLNFDIEPELA
AKIGALKPALEDLITVACDNPSDILRLEEPYAQLVKVVKDLCVKSAGDFG
LQR

3D structure

• PDB: 8b9g Cryo-EM structure of MLE in complex with ADP:AlF4 and U10 RNA
• Chain: A
• Resolution: 2.86 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	A197 R442 R443 R470 G488 V489 R492 K493 V635 S636 F637 G662 W663 N664 H691 S692 Q693 T717 N718 I719 R739 Y752 P863 F894 E896 L900 Q953 Q966 H1032 T1034 T1058	A53 R259 R260 R287 G305 V306 R309 K310 V430 S431 F432 G457 W458 N459 H486 S487 Q488 T512 N513 I514 R534 Y547 P658 F689 E691 L695 Q748 Q761 H827 T829 T853
BS02	ADP	A	G410 C411 G412 K413 T414 T415 R452 T725 R771	G227 C228 G229 K230 T231 T232 R269 T520 R566
BS03	ALF	A	K413 T414 D507	K230 T231 D324

Gene Ontology

Molecular Function

• GO:0001069 regulatory region RNA binding
• GO:0003676 nucleic acid binding
• GO:0003678 DNA helicase activity
• GO:0003682 chromatin binding
• GO:0003690 double-stranded DNA binding
• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0003725 double-stranded RNA binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0033679 3'-5' DNA/RNA helicase activity
• GO:0034458 3'-5' RNA helicase activity
• GO:0043138 3'-5' DNA helicase activity
• GO:0106222 lncRNA binding

Biological Process

• GO:0007549 sex-chromosome dosage compensation
• GO:0008340 determination of adult lifespan
• GO:0009047 dosage compensation by hyperactivation of X chromosome
• GO:0010468 regulation of gene expression
• GO:0031453 positive regulation of heterochromatin formation
• GO:0032508 DNA duplex unwinding
• GO:0042714 dosage compensation complex assembly
• GO:0045433 male courtship behavior, veined wing generated song production
• GO:0045944 positive regulation of transcription by RNA polymerase II
• GO:0048675 axon extension
• GO:0050684 regulation of mRNA processing
• GO:2000373 positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
• GO:2000765 regulation of cytoplasmic translation

Cellular Component

• GO:0000228 nuclear chromosome
• GO:0000785 chromatin
• GO:0000805 X chromosome
• GO:0005634 nucleus
• GO:0005694 chromosome
• GO:0005730 nucleolus
• GO:0005829 cytosol
• GO:0016456 X chromosome located dosage compensation complex, transcription activating
• GO:0072487 MSL complex
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8b9g, PDBe:8b9g, PDBj:8b9g
• PDBsum: 8b9g
• PubMed: 37989319
• UniProt: P24785|MLE_DROME Dosage compensation regulator mle (Gene Name=mle)