Structure of PDB 8att Chain A

Receptor sequence

>8attA (length=906) Species: 559292 (Saccharomyces cerevisiae S288C)

ALNIFNQDRQKVLENRATEAARERWKHDFEEAKARGDISIEKNLNVKLWK
WYNEMLPLVKEEINHCRSLLSEKLSDKKGLNKVDTNRLGYGPYLTLIDPG
KMCVITILELLKLNSTGGVIEGMRTARAVISVGKAIEMEFRSEQVLKSES
QAKILWPQSIRARIGSVLISMLIQVAKVSVQGVDPVTKAKVHGEAPAFAH
GYQYHNGSKLGVLKIHKTLIRQLNGERLIASVQPQLLPMLVEPKPWVNWR
SGGYHYTQSTLLRTKDSPEQVAYLKAASDNGDIDRVYDGLNVLGRTPWTV
NRKVFDVVSQVWNKGEGFLDIPGAQDEMVLPPAPPKNSDPSILRAWKLQV
KTIANKFSSDRSNRCDTNYKLEIARAFLGEKLYFPHNLDFRGRAYPLSPH
FNHLGNDMSRGLLIFWHGKKLGPSGLKWLKIHLSNLFGFDKLPLKDRVAF
TESHLQDIKDSAENPLTGDRWWTTADKPWQALATCFELNEVMKMDNPEEF
ISHQPVHQDGTCNGLQHYAALGGDVEGATQVNLVPSDKPQDVYAHVARLV
QKRLEIAAEKGDENAKILKDKITRKVVKQTVMTNVYGVTYVGATFQIAKQ
LSPIFDDRKESLDFSKYLTKHVFSAIRELFHSAHLIQDWLGESAKRISKS
IRLDVDEKSFKNGNKPDFMSSVIWTTPLGLPIVQPYREESKKQVETNLQT
VFISDPFAVNPVNARRQKAGLPPNFIHSLDASHMLLSAAECGKQGLDFAS
VHDSYWTHASDIDTMNVVLREQFIKLHEVDLVLRLKEEFDQRYKNYVKIG
KLKRSTDLAQKIIRIRKDLSRKLGRSTTLADEIYFEKKRQELLNSPLIED
RNVGEKMVTTVSLFEDITDLDALEGMSVLLPLRLPEIPPKGDFDVTVLRN
SQYFFS

3D structure

• PDB: 8att Structures illustrate step-by-step mitochondrial transcription initiation
• Chain: A
• Resolution: 3.44 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Y640 N642 G643 Y1026 V1027 K1052	Y204 N206 G207 Y590 V591 K616
BS02	dna	A	R530 R533 Y638 S644 K645 G647 R699 R827 T1019 G1023 V1024 T1025 V1027 Y1122 Q1129 Q1135 F1138 R1151 R1152	R124 R127 Y202 S208 K209 G211 R263 R391 T583 G587 V588 T589 V591 Y686 Q693 Q699 F702 R715 R716
BS03	rna	A	L840 G841 R846 M1018 H1188 D1189	L404 G405 R410 M582 H752 D753

Gene Ontology

Molecular Function

• GO:0001018 mitochondrial promoter sequence-specific DNA binding
• GO:0003677 DNA binding
• GO:0003896 DNA primase activity
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0000002 mitochondrial genome maintenance
• GO:0006269 DNA replication, synthesis of primer
• GO:0006351 DNA-templated transcription
• GO:0006390 mitochondrial transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0034245 mitochondrial DNA-directed RNA polymerase complex
• GO:0042645 mitochondrial nucleoid
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:8att, PDBe:8att, PDBj:8att
• PDBsum: 8att
• PubMed: 37821701
• UniProt: P13433|RPOM_YEAST DNA-directed RNA polymerase, mitochondrial (Gene Name=RPO41)