Structure of PDB 8asw Chain A

Receptor sequence

>8aswA (length=1255) Species: 4932 (Saccharomyces cerevisiae)

MRNLITLNKGKFKPTASTAEGDEDDLSFTLLDSVFDTLSDSITCVLGSTD
IGAIEVQQFMKDGSRNVLASFNIQTFDDKLLSFVHFADINQLVFVFEQGD
IITATYDPVSLDPAETLIEIMGTIDNGIAAAQWSYDEETLAMVTKDRNVV
VLSKLFEPISEYHLEVDDLKISKHVDPTMPYMVDTGDVTALDSHEITISW
RGDCDYFAVSSVEEVPDEDDETKSIKRRAFRVFSREGQLDSASEPVTGME
HQLSWKPQGSLIASIQRKTDLGEEDSVDVIFFERNGLRHGEFDTRLPLDE
KVESVCWNSNSEALAVVLANRIQLWTSKNYHWYLKQELYASDISYVKWHP
EKDFTLMFSDAGFINIVDFAYKMAQGPTLEPFDNGTSLVVDGRTVNITPL
ALANVPPPMYYRDFETPGNVLDVACSFSNEIYAAINKDVLIFAAVPSIEE
MKKGKHPSIVCEFPKSEFTSEVDSLRQVAFINDSIVGVLLDTDNLSRIAL
LDIQDITQPTLITIVEVYDKIVLLRSDFDYNHLVYETRDGTVCQLDAEGQ
LMEITKFPQLVRDFRVKRVHNTSAEDDDNWSAESSELVAFGITNNGKLFA
NQVLLASAVTSLEITDSFLLFTTAQHNLQFVHLNSTDFKPLPLVEEGVED
ERVRAIERGSILVSVIPSKSSVVLQATRGNLETIYPRIMVLAEVRKNIMA
KRYKEAFIVCRTHRINLDILHDYAPELFIENLEVFINQIGRVDYLNLFIS
CLSEDDVTKTKYKETLYSGISKSFGMEPAPLTEMQIYMKKKMFDPKTSKV
NKICDAVLNVLLSNPEYKKKYLQTIITAYASQNPQNLSAALKLISELENS
EEKDSCVTYLCFLQDVNVVYKSALSLYDVSLALLVAQKSQMDPREYLPFL
QELQDNEPLRRKFLIDDYLGNYEKALEHLSEIDKDGNVSEEVIDYVESHD
LYKHGLALYRYDSEKQNVIYNIYAKHLSSNQMYTDAAVAYEMLGKLKEAM
GAYQSAKRWREAMSIAVQKFPEEVESVAEELISSLTFEHRYVDAADIQLE
YLDNVKEAVALYCKAYRYDIASLVAIKAKKDELLEEVVDPGLGEGFGIIA
ELLADCKGQINSQLRRLRELRAKKEENPYAFYGQETEQADDVSVAPSETS
TQESFFTRYTGKTGGTAKTGASRRTAKNKRREERKRARGKKGTIYEEEYL
VQSVGRLIERLNQTKPDAVRVVEGLCRRNMREQAHQIQKNFVEVLDLLKA
NVKEI

3D structure

• PDB: 8asw Cryo-EM structure of the fully assembled Elongator complex.
• Chain: A
• Resolution: 3.96 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S1205 E1208 S1209 F1211 T1212 Y1214 G1216 K1217 T1218 G1219 R1236 E1237 R1239 K1240 R1241 R1243	S1150 E1153 S1154 F1156 T1157 Y1159 G1161 K1162 T1163 G1164 R1181 E1182 R1184 K1185 R1186 R1188

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0005515 protein binding
• GO:0042802 identical protein binding

Biological Process

• GO:0002098 tRNA wobble uridine modification
• GO:0002926 tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation
• GO:0006357 regulation of transcription by RNA polymerase II
• GO:0006417 regulation of translation
• GO:0008033 tRNA processing
• GO:0015031 protein transport

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0033588 elongator holoenzyme complex

External links

• PDB: RCSB:8asw, PDBe:8asw, PDBj:8asw
• PDBsum: 8asw
• PubMed: 36617428
• UniProt: Q06706|ELP1_YEAST Elongator complex protein 1 (Gene Name=IKI3)