Structure of PDB 8asg Chain A

Receptor sequence

>8asgA (length=1544) Species: 992210 (SFTS virus AH12)

MNLEVLCGRINVENGLSLGEPGLYDQIYDRPGLPDLDVTVDATGVTVDIG
AVPDSASQLGSSINAGLITIQLSEAYKINHDFTFSGLRRLSEVFPITHDG
SDGMTPAVIHTRLDGTIVVVEFSTTRSHNIGGLEAAYRTKIEKYRDPISR
RVDIMENPRVFFGVIVVSSGGVLSNMPLTQDEAEELMYRFCIANEIYTKA
RSMDADIELQKSEEELEAISRALSFFSLFEPNIERVEGTFPNSEIKMLEQ
FLSTPADVDFITKTLKAKEVEAYADLCDSHYLKPEKTIQERLEINRCEAI
DKTQDLLAGLHARSNKQTSLNRGTVKLPPWLPKPSSESIDIKTDSGFGSL
MDHGAYGELWAKCLLDVSLGNVEGVVSDPAKELDIAISDDPEKDTPKEAK
ITYRRFKPALSSSARQEFSLQGVEGKKWKRMAANQKKEKESHETLSPFLD
VEDIGDFLTFNNLLTDSRYGDESIQRAVSILLEKASAMQDTELTHALNDS
FKRNLSSNVVQWSLWVSCLAQELASALKQHCRAGEFIIKKLKFWPIYVII
KPTKSSSHIFYSLGIRKADVTRRLTGRVFSDTIDAGEWELTEFKSLKTCK
LTNLVNLPCTMLNSIAFWREKLGVAPWLVRKPCSELREQVGLTFLISLED
KSKTEEIITLTRYTQMEGFVSPPMLPKPQKMLGKLDGPLRTKLQVYLLRK
HLDCMVRIASQPFSLIPREGRVEWGGTFHAISGRSTNLENMVNSWYIGYY
KNKEESTELNALGEMYKKIVEMEEDKPSSPEFLGWGDTDSPKKHEFSRSF
LRAACSSLEREIAQRHGRQWKQNLEERVLREIGTKNILDLASMKATSNFS
KDWELYSEVQTKEYHRSKLLEKMATLIEKGVMWYIDAVGQAWKAVLDDGC
MRICLFKKNQHREIYVMDANARLVQFGVETMARCVCELSPHETVANPRLK
NSIIENHGLKSARSLGPGSININSSNDAKKWNQGHYTTKLALVLCWFMPA
KFHRFIWAAISMFRRKKMMVDLRFLAHLSSKSESRSSDPFREAMTDAFHG
NRDVSWMDKGRTYIKTETGMMQGILHFTSSLLHSCVQSFYKSYFVSKLKE
GYMGESISGVVDVIEGSDDSAIMISIRPKSDMDEVRSRFFVANLLHSVKF
LNPLFGIYSSEKSTVNTVYCVEYNSEFHFHRHLVRPTLRWIAASHQISET
EALASRQEDYSNLLTQCLEGGASFSLTYLIQCAQLLHHYMLLGLCLHPLF
GTFMGMLISDPDPALGFFLMDNPAFAGGAGFRFNLWRACKTTDLGRKYAY
YFNEIQGHSVMVYWGDRKKYQALLNRMGLPEDWVEQIDENPGVLYRRAAN
KKELLLKLAEKVHSPGVTSSLSKGHVVPRVVAAGVYLLSRHCFRFSGSTQ
KASLIKLLMMSSISAMKHGGSLNPNQERMLFPQAQEYDRVCTLLEEVEHL
TGKFVVRERNIVRSRIDLFAEDLVSEVWFGEWDKLRASFAWLSTDPSETL
RDGPFLSHVQFRNFIAHRSVRLLGAPVTISQVVRMNFFPGFSLE

3D structure

• PDB: 8asg Structural insights into viral genome replication by the severe fever with thrombocytopenia syndrome virus L protein.
• Chain: A
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K849 A850 R920 I922 Y923 R930 Q933 Q1080 G1081	K844 A845 R912 I914 Y915 R922 Q925 Q1072 G1073
BS02	rna	A	R318 K331 G430 K431 K442 E443 S446 H447 T558 F565 S600 K602 K605 K656 R695 E763 L764 N765 H1035 L1036 R1043 S1045 R1049 T1053	R313 K326 G425 K426 K437 E438 S441 H442 T553 F560 S595 K597 K600 K651 R690 E758 L759 N760 H1027 L1028 R1035 S1037 R1041 T1045
BS03	rna	A	K386 E387 K405 Y408 S530 K533 Q534 K544 L1254 V1340 R1345 V1413 Y1414 R1418 C1420	K381 E382 K400 Y403 S525 K528 Q529 K539 L1246 V1312 R1317 V1385 Y1386 R1390 C1392
BS04	MG	A	D985 D1127 E1180	D977 D1119 E1172

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:8asg, PDBe:8asg, PDBj:8asg
• PDBsum: 8asg
• PubMed: 36651274
• UniProt: U3GU88