Structure of PDB 8as7 Chain A

Receptor sequence

>8as7A (length=1575) Species: 992210 (SFTS virus AH12)

MNLEVLCGRINVENGLSLGEPGLYDQIYDRPGLPDLDVTVDATGVTVDIG
AVPDSASQLGSSINAGLITIQLSEAYKINHDFTFSGLSKTTDRRLSEVFP
ITHDGSDGMTPAVIHTRLDGTIVVVEFSTTRSHNIGGLEAAYRTKIEKYR
DPISRRVDIMENPRVFFGVIVVSSGGVLSNMPLTQDEAEELMYRFCIANE
IYTKARSMDADIELQKSEEELEAISRALSFFSLFEPNIERVEGTFPNSEI
KMLEQFLSTPADVDFITKTLKAKEVEAYADLCDSHYLKPEKTIQERLEIN
RCEAIDKTQDLLAGLHARSNKQTSLNRGTVKLPPWLPKPSSESIDIKTDS
GFGSLMDHGAYGELWAKCLLDVSLGNVEGVVSDPAKELDIAISAKITYRR
FKPALSSSARQEFSLQGVEGKKWKRMAANQKKEKESHETLSPFLDVEDIG
DFLTFNNLLTDSRYGDESIQRAVSILLEKASAMQDTELTHALNDSFKRNL
SSNVVQWSLWVSCLAQELASALKQHCRAGEFIIKKLKFWPIYVIIKPTKS
SSHIFYSLGIRKADVTRRLTGRVFSDTIDAGEWELTEFKSLKTCKLTNLV
NLPCTMLNSIAFWREKLGVAPWLVRKPCSELREQVGLTFLISLEDKSKTE
EIITLTRYTQMEGFVSPPMLPKPQKMLGKLDGPLRTKLQVYLLRKHLDCM
VRIASQPFSLIPREGRVEWGGTFHAISGRSTNLENMVNSWYIGYYKNKEE
STELNALGEMYKKIVEMEEDKPSSPEFLGWGDTDSPKKHEFSRSFLRAAC
SSLEREIAQRHGRQWKQNLEERVLREIGTKNILDLASMKATSNFSKDWEL
YSEVQTKEYHRSKLLEKMATLIEKGVMWYIDAVGQAWKAVLDDGCMRICL
FKKNQHGGLREIYVMDANARLVQFGVETMARCVCELSPHETVANPRLKNS
IIENHGLKSARSLGPGSININSSNDAKKWNQGHYTTKLALVLCWFMPAKF
HRFIWAAISMFRRKKMMVDLRFLAHLSSKSESRSSDPFREAMTDAFHGNR
DVSWMDKGRTYIKTETGMMQGILHFTSSLLHSCVQSFYKSYFVSKLKEGY
MGESISGVVDVIEGSDDSAIMISIRPKSDMDEVRSRFFVANLLHSVKFLN
PLFGIYSSEKSTVNTVYCVEYNSEFHFHRHLVRPTLRWIAASHQISETEA
LASRQEDYSNLLTQCLEGGASFSLTYLIQCAQLLHHYMLLGLCLHPLFGT
FMGMLISDPDPALGFFLMDNPAFAGGAGFRFNLWRACKTTDLGRKYAYYF
NEIQGKGTLSHSVMVYWGDRKKYQALLNRMGLPEDWVEQIDENPGVLYRR
AANKKELLLKLAEKVHSPGVTSSLSKGHVVPRVVAAGVYLLSRHCFRFSQ
KASLIKLLMMSSISAMKHGGSLNPNQERMLFPQAQEYDRVCTLLEEVEHL
TGKFVVRERNIVRSRIDLFQEPVDLRCKAEDLVSEVWFGLKRTKLGPRLL
KEEWDKLRASFAWLSTDPSETLRDGPFLSHVQFRNFIAHVDAKSRSVRLL
GAPVKKVTTISQVVRMNFFPGFSLE

3D structure

• PDB: 8as7 Structural insights into viral genome replication by the severe fever with thrombocytopenia syndrome virus L protein.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R318 K331 Q426 G427 E429 K431 E443 K444 S446 H447 T449 H535 T558 K559 S562 H563 F565 S600 K602 K605 K656 R695 E763 L764 N765 F1032 H1035 S1044 S1045 R1049	R318 K331 Q416 G417 E419 K421 E433 K434 S436 H437 T439 H525 T548 K549 S552 H553 F555 S590 K592 K595 K646 R685 E753 L754 N755 F1022 H1025 S1034 S1035 R1039
BS02	rna	A	K533 H535 R537 S560 S561 K849 A850 H870 R871 F911 K913 N914 I922 Y923 R930 V952 P955 R1031 Q1080 G1081 F1085 W1342 G1343 R1345 K1346 K1347 Y1348 K1401 G1402 H1403 R1407	K523 H525 R527 S550 S551 K839 A840 H860 R861 F901 K903 N904 I912 Y913 R920 V942 P945 R1021 Q1070 G1071 F1075 W1317 G1318 R1320 K1321 K1322 Y1323 K1376 G1377 H1378 R1382
BS03	rna	A	Y754 N1182 R1197 W1198 A1201 Q1224	Y744 N1172 R1187 W1188 A1191 Q1214
BS04	MG	A	A986 D1126	A976 D1116
BS05	MG	A	D985 D1127	D975 D1117
BS06	2KH	A	R920 A986 N990 Q1080 G1081 D1126 K1170	R910 A976 N980 Q1070 G1071 D1116 K1160

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:8as7, PDBe:8as7, PDBj:8as7
• PDBsum: 8as7
• PubMed: 36651274
• UniProt: U3GU88