Structure of PDB 8aq4 Chain A

Receptor sequence

>8aq4A (length=509) Species: 83333 (Escherichia coli K-12)

MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFN
RRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAY
LSLYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQ
FGYSQIDGPLKGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVV
LFALPFPLRYIQWFTPQPEKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYY
NATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIV
DARLNKQNRYDTYNTIITLGKGAPYSYESADRYNKNHLVPFGEFVPLESI
LRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIILGEQVRDNF
RPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGF
AAVLMSLRQ

3D structure

• PDB: 8aq4 Structure snapshots reveal the mechanism of a bacterial membrane lipoprotein N -acyltransferase.
• Chain: A
• Resolution: 2.62 Å

Enzymatic activity

• Enzyme Commision number: 2.3.1.269: apolipoprotein N-acyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	6OU	A	A22 S29 P30 Y31 D32 K418 W423 F426 Q427 R430	A22 S29 P30 Y31 D32 K418 W423 F426 Q427 R430
BS02	QGT	A	L143 F146 V339 C387 F416	L143 F146 V339 C387 F416
BS03	6OU	A	S78 Y100 K236 W237 E267 S268 G342 F357 P361 M362 C387 N412 W415	S78 Y100 K236 W237 E267 S268 G342 F357 P361 M362 C387 N412 W415
BS04	6OU	A	F137 N488 W492	F137 N488 W492

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0016410 N-acyltransferase activity
• GO:0016746 acyltransferase activity

Biological Process

• GO:0042158 lipoprotein biosynthetic process

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0030288 outer membrane-bounded periplasmic space

External links

• PDB: RCSB:8aq4, PDBe:8aq4, PDBj:8aq4
• PDBsum: 8aq4
• PubMed: 37390210
• UniProt: P23930|LNT_ECOLI Apolipoprotein N-acyltransferase (Gene Name=lnt)