Structure of PDB 8afj Chain A

Receptor sequence
>8afjA (length=197) Species: 160488 (Pseudomonas putida KT2440) [Search protein sequence]
IPEIDAFLGCPTPDAWIEAALADQETLLIDHKNCEFKAASTALSLIAKYN
THLDLINMMSRLAREELVHHEQVLRLMKRRGVPLRPVSAGRYASGLRRLV
RAHEPVKLVDTLVVGAFIEARSCERFAALVPHLDEELGRFYHGLLKSEAR
HYQGYLKLAHNYGDEADIARRVELVRAAEMELIQSPDQELRFHSGIP
3D structure
PDB8afj tRNA modifying enzyme MiaE soaked in Na-dithionite in a glovebox and flash-cooled using a miniature-airlock
ChainA
Resolution1.6 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FE A E38 E69 H72 E35 E66 H69
BS02 FE A E69 E122 E151 H154 E66 E119 E148 H151
Gene Ontology
Molecular Function
GO:0045301 tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine)-hydroxylase activity
GO:0046872 metal ion binding
Biological Process
GO:0006400 tRNA modification

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Molecular Function
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Biological Process
External links
PDB RCSB:8afj, PDBe:8afj, PDBj:8afj
PDBsum8afj
PubMed
UniProtA0A179QS89

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