Structure of PDB 8ack Chain A

Receptor sequence

>8ackA (length=468) Species: 287 (Pseudomonas aeruginosa)

SICKSPLLVSTPLGLPRCLQASNVVKRLQKLEDIASLNDGNRAAATPGYQ
ASVDYVKQTLQKAGYKVSVQPFPFTAYYPKGPGSLSATVPQPVTYEWEKD
FTYLSQTEAGDVTAKVVPVDLSLGAGNTSTSGCEAEDFANFPAGSIALIQ
RGTCNFEQKAENAAAAGAAGVIIFNQGNTDDRKGLENVTVGESYEGGIPV
IFATYDNGVAWSQTPDLQLHLVVDVVRKKTETYNVVAETRRGNPNNVVMV
GAHLDSVFEGPGINDNGSGSAAQLEMAVLLAKALPVNKVRFAWWGAEEAG
LVGSTHYVQNLAPEEKKKIKAYLNFDMIGSPNFGNFIYDGDGSDFGLQGP
PGSAAIERLFEAYFRLRGQQSEGTEIDFRSDYAEFFNSGIAFGGLFTGAE
GLKTEEQAQKYGGTAGKAYDECYHSKCDGIANINQDALEIHSDAMAFVTS
WLSLSTKVVDDEIAAAGQ

3D structure

• PDB: 8ack An anti-biofilm cyclic peptide targets a secreted aminopeptidase from P. aeruginosa.
• Chain: A
• Resolution: 1.784 Å

Enzymatic activity

• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	R194 T196 C197 N198 F199 N230 V231 T232 G343 L344 F421 R422 A426 E427 N430	R151 T153 C154 N155 F156 N187 V188 T189 G300 L301 F378 R379 A383 E384 N387
BS02	ZN	A	D308 E341 H467	D265 E298 H424
BS03	ZN	A	H296 D308 D369	H253 D265 D326
BS04	ZN	A	E482 D486	E439 D443
BS05	ZN	A	D382 D384 S386 E400	D339 D341 S343 E357

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0008235 metalloexopeptidase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0015628 protein secretion by the type II secretion system
• GO:0043952 protein transport by the Sec complex

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:8ack, PDBe:8ack, PDBj:8ack
• PDBsum: 8ack
• PubMed: 37386135
• UniProt: Q9HZQ8|LAP_PSEAE Aminopeptidase (Gene Name=lap)