Structure of PDB 8a3w Chain A

Receptor sequence

>8a3wA (length=254) Species: 347515 (Leishmania major strain Friedlin)

GKTVLSCRKGNGSVYQVHGHKRLGPAKLRILDYAERHGYMRGVVKSIEHE
AGRGAALARVEFRHPYKFRRVKELMVAPEGMFTGQSVFCGQKAPLAIGNV
LPLGQITEGCIVCNVEAKPGDRGTLARASGDYCIIISHNHETGRTRLKLP
SGQKKSVPSTSRAMIGIISGGGRIEKPVLKAGNSFYRFRGKRNCWPKVRG
VARNPVEHPHGGGNHQHIGHPSTVSRHSPPGQKVGLIAARRTGRIRGGKA
VKGA

3D structure

• PDB: 8a3w CRYO-EM STRUCTURE OF LEISHMANIA MAJOR 80S RIBOSOME : WILD TYPE
• Chain: A
• Resolution: 2.89 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	G248 G249	G247 G248
BS02	rna	A	G2 G11 N12 V18 G20 R23 G25 Y34 Y40 R42 K46 R54 Y67 F69 F83 G85 Q86 S87 K93 P95 T125 L126 A127 R128 A129 S130 D132 L150 P151 S152 K156 G172 R174 V179 L180 S185 R193 W196 P197 R200 G201 V202 H211 G213 G214 N215 H216 H218 I219 G220 H221 P222 S223 T224 R227 H228 P230 Q233 G236 I238 A240 R241 R242 T243 G244 R245 G254 A255	G1 G10 N11 V17 G19 R22 G24 Y33 Y39 R41 K45 R53 Y66 F68 F82 G84 Q85 S86 K92 P94 T124 L125 A126 R127 A128 S129 D131 L149 P150 S151 K155 G171 R173 V178 L179 S184 R192 W195 P196 R199 G200 V201 H210 G212 G213 N214 H215 H217 I218 G219 H220 P221 S222 T223 R226 H227 P229 Q232 G235 I237 A239 R240 R241 T242 G243 R244 G253 A254
BS03	rna	A	H50 F69 R71 K177 Y187 R188	H49 F68 R70 K176 Y186 R187
BS04	rna	A	R9 S14 H19 H21 A182 F186 Y187 W196 V199 R204 N205 V207 E208	R8 S13 H18 H20 A181 F185 Y186 W195 V198 R203 N204 V206 E207

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8a3w, PDBe:8a3w, PDBj:8a3w
• PDBsum: 8a3w
• UniProt: Q4FWX5