Structure of PDB 7zd2 Chain A

Receptor sequence

>7zd2A (length=461) Species: 208964 (Pseudomonas aeruginosa PAO1)

GFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKILG
CIHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFAW
KGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLERI
HGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHSLN
DAIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPICAM
QACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCDANM
LKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGFD
AHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYAD
LPAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGVSV
EGPFKPDTYRY

3D structure

• PDB: 7zd2 Crystal structure of Pseudomonas aeruginosa S-adenosyl-L-homocysteine hydrolase inhibited by Co2+ ions.
• Chain: A
• Resolution: 2.16 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	T165 T166 T167 N199 I227 G230 D231 V232 E251 V252 T298 N300 V303 I321 H323 N375 H382	T157 T158 T159 N191 I219 G222 D223 V224 E243 V244 T290 N292 V295 I313 H315 N367 H374
BS02	ADN	A	H61 T63 D139 E164 T165 K194 D198 H323 L373 L376 H382 M387	H53 T55 D131 E156 T157 K186 D190 H315 L365 L368 H374 M379
BS03	CO	A	H61 C85 D139 G140 H323	H53 C77 D131 G132 H315
BS04	CO	A	H170 D174	H162 D166
BS05	NAD	A	K463 Y467	K455 Y459

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7zd2, PDBe:7zd2, PDBj:7zd2
• PDBsum: 7zd2
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)