Structure of PDB 7z8h Chain A

Receptor sequence

>7z8hA (length=1083) Species: 9606 (Homo sapiens)

LSIQMANAKFNYGFEYLGVQDKLVQTPLTDRCYLTMTQALEARLGGSPFG
PAGTGKTESVKALGHQLGRFVLVFNCDETFDFQAMGRIFVGLCQVGAWGC
FDEFNRLEERMLSAVSQQVQCIQEALREHSNPNYDKTSAPITCELLNKQV
KVSPDMAIFITMNPGSNLPDNLKKLFRSLAMTKPDRQLIAQVMLYSQGFR
TAEVLANKIVPFFKLCDEQLSSQSHYDFGLRALKSVLVSAGNVKRERIQK
IKREKEERGEAVDEGEIAENLPEQEILIQSVCETMVPKLVAEDIPLLFSL
LSDVFPGVQYHRGEMTALREELKKVCQEMYLTYGDGEEVGGMWVEKVLQL
YQITQINHGLMMVGPSGSGKSMAWRVLLKALERLEGVEGVAHIIDPKAIS
KDHLYGTLDPNTREWTDGLFTHVLRKIIDSVRGELQKRQWIVFDGDVDPE
WVENLNSVLDDNKLLTLPNGERLSLPPNVRIMFEVQDLKYATLATVSRCG
MVWFSEDVLSTDMIFNNFLARLRSIPLDEGEDEAQRRRKGKEDEGEEAAS
PMLQIQRDAATIMQPYFTSNGLVTKALEHAFQLEHIMDLTRLRCLGSLFS
MLHQACRNVAQYNANHPDFPMQIEQLERYIQRYLVYAILWSLSGDSRLKM
RAELGEYIRRITTVPLPTAPNIPIIDYEVSISGEWSPWQAKVPQIEVETH
KVAAPDVVVPTLDTVRHEALLYTWLAEHKPLVLCGPPGSGKTMTLFSALR
ALPDMEVVGLNFSSATTPELLLKTFDHYCEYRRTPNGVVLAPVQLGKWLV
LFCDEINLPDMDKYGTQRVISFIRQMVEHGGFYRTSDQTWVKLERIQFVG
ACNPPTDPGRKPLSHRFLRHVPVVYVDYPGPASLTQIYGTFNRAMLRLIP
SLRTYAEPLTAAMVEFYTMSQERFTQDTQPHYIYSPREMTRWVRGIFEAL
RPLETLPVEGLIRIWAHEALRLFQDRLVEDEERRWTDENIDTVALKHFPN
IDREKAMSRPILYSNWLSKDYIPVDQEELRDYVKARLKVFYEEELDVPLV
LFNEVLDHVLRIDRIFRQPQGHTLSRFVAWMNG

3D structure

• PDB: 7z8h Structure of dynein-dynactin on microtubules shows tandem adaptor binding.
• Chain: A
• Resolution: 3.41 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	A	L1879 V1880 G1909 T1910 G1911 K1912 T1913 E1914 I2049 L2090 K2094	L23 V24 G53 T54 G55 K56 T57 E58 I189 L230 K234
BS02	ATP	A	L2191 T2192 W2203 S2226 G2227 S2228 G2229 K2230 S2231 M2232 L2369 M2373 N2377 L2452 R2726 R2729	L331 T332 W343 S366 G367 S368 G369 K370 S371 M372 L509 M513 N517 L592 R866 R869
BS03	ANP	A	V2568 V2569 G2598 S2599 G2600 K2601 T2602 M2603 I2747 R2797 T2800	V708 V709 G738 S739 G740 K741 T742 M743 I887 R937 T940
BS04	MG	A	S2231 E2344 R2726	S371 E484 R866

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement
• GO:0007052 mitotic spindle organization
• GO:0007097 nuclear migration
• GO:0008090 retrograde axonal transport
• GO:0031122 cytoplasmic microtubule organization
• GO:0032388 positive regulation of intracellular transport
• GO:0033962 P-body assembly
• GO:0034063 stress granule assembly
• GO:0051293 establishment of spindle localization
• GO:0051301 cell division
• GO:0060236 regulation of mitotic spindle organization
• GO:0090235 regulation of metaphase plate congression
• GO:0120162 positive regulation of cold-induced thermogenesis
• GO:1905832 positive regulation of spindle assembly

Cellular Component

• GO:0005576 extracellular region
• GO:0005737 cytoplasm
• GO:0005813 centrosome
• GO:0005829 cytosol
• GO:0005856 cytoskeleton
• GO:0005868 cytoplasmic dynein complex
• GO:0005874 microtubule
• GO:0005881 cytoplasmic microtubule
• GO:0005938 cell cortex
• GO:0016020 membrane
• GO:0030175 filopodium
• GO:0030286 dynein complex
• GO:0035578 azurophil granule lumen
• GO:0070062 extracellular exosome
• GO:1904115 axon cytoplasm

External links

• PDB: RCSB:7z8h, PDBe:7z8h, PDBj:7z8h
• PDBsum: 7z8h
• PubMed: 36071160
• UniProt: Q14204|DYHC1_HUMAN Cytoplasmic dynein 1 heavy chain 1 (Gene Name=DYNC1H1)