Structure of PDB 7z8g Chain A

Receptor sequence

>7z8gA (length=3047) Species: 9606 (Homo sapiens)

QDLKGVWSELSKVWEQIDQMKEQPWVSVQPRKLRQNLDALLNQLKSFPAR
LRQYASYEFVQRLLKGYMKINMLVIELKSEALKDRHWKQLMKRLHVNWVV
SELTLGQIWDVDLQKNEAIVKDVLLVAQGEMALEEFLKQIREVWNTYELD
LVNYQNKCRLIRGWDDLFNKVKEHINSVSAMKLSPYYKVFEEDALSWEDK
LNRIMALFDVWIDVQRRWVYLEGIFTGSADIKHLLPVETQEFQSISTEFL
ALMKKVSKSPLVMDVLNIQGVQRSLERLADLLGEIQKALGEYLERERSSF
PRFYFVGDEDLLEIIGNSKNVAKLQKHFKKMFAGVSSIILNEDNSVVLGI
SSREGEEVMFKTPVSITEHPKINEWLTLVEKEMRVTLAKLLAESVTEVEI
FGKATSIDPNTYITWIDKYQAQLVVLSAQIAWSENVETALSSMGGGGDAA
PLHSVLSNVEVTLNVLADSVLMEQPPLRRRKLEHLITELVHQRDVTRSLI
KSKIDNAKSFEWLSQMRFYFDPKQTDVLQQLSIQMANAKFNYGFEYLGVQ
DKLVQTPLTDRCYLTMTQALEARLGGSPFGPAGTGKTESVKALGHQLGRF
VLVFNCDETFDFQAMGRIFVGLCQVGAWGCFDEFNRLEERMLSAVSQQVQ
CIQEALREHSNPNYDKTSAPITCELLNKQVKVSPDMAIFITMNPGSNLPD
NLKKLFRSLAMTKPDRQLIAQVMLYSQGFRTAEVLANKIVPFFKLCDEQL
SSQSHYDFGLRALKSVLVSAGNVKRERIQKIKREKEERGEAVDEGEIAEN
LPEQEILIQSVCETMVPKLVAEDIPLLFSLLSDVFPGVQYHRGEMTALRE
ELKKVCQEMYLTYGDGEEVGGMWVEKVLQLYQITQINHGLMMVGPSGSGK
SMAWRVLLKALERLEGVEGVAHIIDPKAISKDHLYGTLDPNTREWTDGLF
THVLRKIIDSVRGELQKRQWIVFDGDVDPEWVENLNSVLDDNKLLTLPNG
ERLSLPPNVRIMFEVQDLKYATLATVSRCGMVWFSEDVLSTDMIFNNFLA
RLRSIPLDEGEDEAQRRRKGKEDEGEEAASPMLQIQRDAATIMQPYFTSN
GLVTKALEHAFQLEHIMDLTRLRCLGSLFSMLHQACRNVAQYNANHPDFP
MQIEQLERYIQRYLVYAILWSLSGDSRLKMRAELGEYIRRITTVPLPTAP
NIPIIDYEVSISGEWSPWQAKVPQIEVETHKVAAPDVVVPTLDTVRHEAL
LYTWLAEHKPLVLCGPPGSGKTMTLFSALRALPDMEVVGLNFSSATTPEL
LLKTFDHYCEYRRTPNGVVLAPVQLGKWLVLFCDEINLPDMDKYGTQRVI
SFIRQMVEHGGFYRTSDQTWVKLERIQFVGACNPPTDPGRKPLSHRFLRH
VPVVYVDYPGPASLTQIYGTFNRAMLRLIPSLRTYAEPLTAAMVEFYTMS
QERFTQDTQPHYIYSPREMTRWVRGIFEALRPLETLPVEGLIRIWAHEAL
RLFQDRLVEDEERRWTDENIDTVALKHFPNIDREKAMSRPILYSNWLSKD
YIPVDQEELRDYVKARLKVFYEEELDVPLVLFNEVLDHVLRIDRIFRQPQ
GHLLLIGVSGAGKTTLSRFVAWMNGLSVYQIKVHRKYTGEDFDEDLRTVL
RRSGCKNEKIAFIMDESNVLDSGFLERMNTLLANGEVPGLFEGDEYATLM
TQCKEGAQKEGLMLDSHEELYKWFTSQVIRNLHVVFTMNPSSEGLKDRAA
TSPALFNRCVLNWFGDWSTEALYQVGKEFTSKMDLEKPNYIVPDYMPVVY
DKLPQPPSHREAIVNSCVFVHQTLHQANARLAKRGGRTMAITPRHYLDFI
NHYANLFHEKRSELEEQQMHLNVGLRKIKETVDQVEELRRDAKVNRSTAL
LKSLSAERERWEKTSETFKNQMSTIAGDCLLSAAFIAYAGYFDQQMRQNL
FTTWSHHLQQANIQFRTDIARTEYLSNADERLRWQASSLPADDLCTENAI
MLKRFNRYPLIIDPSGQATEFIMNEYKDRKITRTSFLDDAFRKNLESALR
FGNPLLVQDVESYDPVLNPVLNREVRRTGGRVLITLGDQDIDLSPSFVIF
LSTRDPTVEFPPDLCSRVTFVNFTVTRSSLQSQCLNEVLKAERPDVDEKR
SDLLKLQGEFQLRLRQLEKSLLQALNEVKGRILDDDTIITTLENLKREAA
EVTRKVEETDIVMQEVETVSQQYLPLSTACSSIYFTMESLKQIHFLYQYS
LQFFLDIYHNVLYENPNLKGVTDHTQRLSIITKDLFQVAFNRVARGMLHQ
DHITFAMLLARIKLKGTVGEPTYDAEFQHFLRGNEIVLSAGSTPRIQGLT
VEQAEAVVRLSCLPAFKDLIAKVQADEQFGIWLDSSSPEQTVPYLWSEET
PATPIGQAIHRLLLIQAFRPDRLLAMAHMFVSTNLGESFMSIMEQPLDLT
HIVGTEVKPNTPVLMCSVPGYDASGHVEDLAAEQNTQITSIAIGSAEGFN
QADKAINTAVKSGRWVMLKNVHLAPGWLMQLEKKLHSLQPHACFRLFLTM
EINPKVPVNLLRAGRIFVFEPPPGVKANMLRTFSSIPVSRICKSPNERAR
LYFLLAWFHAIIQERLRYAPLGWSKKYEFGESDLRSACDTVDTWLDDTAK
GRQNISPDKIPWSALKTLMAQSIYGGRVDNEFDQRLLNTFLERLFTTRSF
DSEFKLACKVDGHKDIQMPDGIRREEFVQWVELLPDTQTPSWLGLPNNAE
RVLLTTQGVDMISKMLKMQMLEDEDAWMRTLHTTASNWLHLIPQTLSHLK
RTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLR
TLINELVKGILPRSWSHYTVPAGMTVIQWVSDFSERIKQLQNISLAAASG
GAKELKNIHVCLGGLFVPEAYITATRQYVAQANSWSLEELCLEVNVTTSQ
GATLDACSFGVTGLKLQGATCNNNKLSLSNAISTALPLTQLRWVKQTNTE
KKASVVTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE

3D structure

• PDB: 7z8g Structure of dynein-dynactin on microtubules shows tandem adaptor binding.
• Chain: A
• Resolution: 3.52 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	A	L1879 V1880 G1909 T1910 G1911 K1912 T1913 E1914 I2049 L2090 R2091 K2094	L553 V554 G583 T584 G585 K586 T587 E588 I719 L760 R761 K764
BS02	ATP	A	L2191 T2192 W2203 S2226 G2227 S2228 G2229 K2230 S2231 M2232 L2369 M2373 N2377 L2452 R2726 R2729	L861 T862 W873 S896 G897 S898 G899 K900 S901 M902 L1039 M1043 N1047 L1122 R1396 R1399
BS03	MG	A	S2231 E2344 R2726	S901 E1014 R1396
BS04	ANP	A	V2568 V2569 G2598 G2600 K2601 T2602 M2603 I2747 R2797 T2800 R3088	V1238 V1239 G1268 G1270 K1271 T1272 M1273 I1417 R1467 T1470 R1758
BS05	ANP	A	L2909 V2910 G2940 G2942 K2943 T2944 T2945 L3177 R3695	L1579 V1580 G1610 G1612 K1613 T1614 T1615 L1847 R2117

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement
• GO:0007052 mitotic spindle organization
• GO:0007097 nuclear migration
• GO:0008090 retrograde axonal transport
• GO:0031122 cytoplasmic microtubule organization
• GO:0032388 positive regulation of intracellular transport
• GO:0033962 P-body assembly
• GO:0034063 stress granule assembly
• GO:0051293 establishment of spindle localization
• GO:0051301 cell division
• GO:0060236 regulation of mitotic spindle organization
• GO:0090235 regulation of metaphase plate congression
• GO:0120162 positive regulation of cold-induced thermogenesis
• GO:1905832 positive regulation of spindle assembly

Cellular Component

• GO:0005576 extracellular region
• GO:0005737 cytoplasm
• GO:0005813 centrosome
• GO:0005829 cytosol
• GO:0005856 cytoskeleton
• GO:0005868 cytoplasmic dynein complex
• GO:0005874 microtubule
• GO:0005881 cytoplasmic microtubule
• GO:0005938 cell cortex
• GO:0016020 membrane
• GO:0030175 filopodium
• GO:0030286 dynein complex
• GO:0035578 azurophil granule lumen
• GO:0070062 extracellular exosome
• GO:1904115 axon cytoplasm

External links

• PDB: RCSB:7z8g, PDBe:7z8g, PDBj:7z8g
• PDBsum: 7z8g
• PubMed: 36071160
• UniProt: Q14204|DYHC1_HUMAN Cytoplasmic dynein 1 heavy chain 1 (Gene Name=DYNC1H1)