Structure of PDB 7z0t Chain A

Receptor sequence

>7z0tA (length=714) Species: 83333 (Escherichia coli K-12)

MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLKGYYGWD
FINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGP
DAIQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVCHGPSVAGLHQ
SVGNGAMSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTE
GFEEYRKIVEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVT
QFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRGQNNVQGACDMGALPDT
YPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAHGEVRAAYI
MGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQE
IWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDT
PNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAA
LADEPGYAQINTEDAKRLGIEDEALVWVHSRKGKIITRAQVSDRPNKGAI
YMTYQWWIGACNELVTENLSPITKTPEYKYCAVRVEPIADQRAAEQYVID
EYNKLKTRLREAAL

3D structure

• PDB: 7z0t Structure of the membrane-bound formate hydrogenlyase complex from Escherichia coli.
• Chain: A
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 1.17.98.4: formate dehydrogenase (acceptor).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	C8 C11 C15 L41 C42 K44 G45 I183	C8 C11 C15 L41 C42 K44 G45 I183
BS02	MGD	A	R110 G111 T112 V139 U140 Q301 Q335 G402 E403 D404 T408 D429 I430 S445 D478 T579 R581 S587 C588 Y654 N662 Y678	R110 G111 T112 V139 U140 Q301 Q335 G402 E403 D404 T408 D429 I430 S445 D478 T579 R581 S587 C588 Y654 N662 Y678
BS03	MGD	A	G174 Y175 N176 S180 D202 P203 R204 N220 G221 N223 G296 M297 F302 G334 V580 R581 E582 H585 Y586 S587 Q655 K679	G174 Y175 N176 S180 D202 P203 R204 N220 G221 N223 G296 M297 F302 G334 V580 R581 E582 H585 Y586 S587 Q655 K679

Gene Ontology

Molecular Function

• GO:0003954 NADH dehydrogenase activity
• GO:0005515 protein binding
• GO:0008863 formate dehydrogenase (NAD+) activity
• GO:0016491 oxidoreductase activity
• GO:0016903 oxidoreductase activity, acting on the aldehyde or oxo group of donors
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006007 glucose catabolic process
• GO:0009061 anaerobic respiration
• GO:0015942 formate metabolic process
• GO:0015944 formate oxidation
• GO:0019628 urate catabolic process
• GO:0019645 anaerobic electron transport chain
• GO:0045333 cellular respiration

Cellular Component

• GO:0005829 cytosol
• GO:0009326 formate dehydrogenase complex
• GO:0016020 membrane
• GO:0045271 respiratory chain complex I
• GO:1990204 oxidoreductase complex

External links

• PDB: RCSB:7z0t, PDBe:7z0t, PDBj:7z0t
• PDBsum: 7z0t
• PubMed: 36104349
• UniProt: P07658|FDHF_ECOLI Formate dehydrogenase H (Gene Name=fdhF)