Structure of PDB 7ync Chain A

Receptor sequence

>7yncA (length=1536) Species: 45657 (Desulfonema ishimotonii)

MTTTMKISIEFLEPFRMTKWQESTRRNKNNKEFVRGQAFARWHRNKKDNT
KGRPYITGTLLRSAVIRSAENLLTLSDGKISEKTCCPGKFDTEDKDRLLQ
LRQRSTLRWTDKNPCPDNAETYCPFCELLGRRFRIHFGNLSLPGKPDFDG
PKAIGSQRVLNRVDFKSGKAHDFFKAYEVDHTRFPRFEGEITIDNKVSAE
ARKLLCDSLKFTDRLCGALCVIRFDNLAEKTAEQIISILDDNKKTEYTRL
LADAIRSLRRSSKLVAGLPKDHDGKDDHYLWDIGKKKKDENSVTIRQILT
TSADTKELKNAGKWREFCEKLGEALYLKSKDMDAEFHGKPDRLEKSRSVS
IGSVLKETVVCGELVAKTPFFFGAIDEDAKQTDLQVLLTPDNKYRLPRSA
VRGILRRDLQTYFDSPCNAELGGRPCMCKTCRIMRGITVMDARSEYNAPP
EIRHRTRINPFTGTVAEGALFNMEVAPEGIVFPFQLRYRGSEDGLPDALK
TVLKWWAEGQAFMSGAASTGKGRFRMENAKYETLDLSDENQRNDYLKNWG
WRDEKGLEELKKRLNSGLPEPGNYRDPKWHEINVSIEMASPFINGDPIRA
AVDKRGTDVVTFVKYKAEGEEAKPVCAYKAESFRGVIRSAVARIHMEDGV
PLTELTHSDCECLLCQIFGSEYEAGKIRFEDLVFESDPEPVTFDHVAIDR
FTGGAADKKKFDDSPLPGSPARPLMLKGSFWIRRDVLEDEEYCKALGKAL
ADVNNGLYPLGGKSAIGYGQVKSLGIKGDDKRISRLMNPAFDETDVAVPE
KPKTDAEVRIEAEKVYYPHYFVEPHKKVEREEKPCGHQKFHEGRLTGKIR
CKLITKTPLIVPDTSNDDFFRPADKEARKEKDEYHKSYAFFRLHKQIMIP
GSELRGMVSSVYETVTNSCFRIFDETKRLSWRMDADHQNVLQDFLPGRVT
ADGKHIQKFSETARVPFYDKTQKHFDILDEQEIAGEKPVRMWVKRFIKRL
SLVDPAKHPQKKQDNKWKRRKEGIATFIEQKNGSYYFNVVTNNGCTSFHL
WHKPDNFDQEKLEGIQNGEKLDCWVRDSRYQKAFQEIPENDPDGWECKEG
YLHVVGPSKVEFSDKKGDVINNFQGTLPSVPNDWKTIRTNDFKNRKRKNE
PVFCCEDDKGNYYTMAKYCETFFFDLKENEEYEIPEKARIKYKELLRVYN
NNPQAVPESVFQSRVARENVEKLKSGDLVYFKHNEKYVEDIVPVRISRTV
DDRMIGKRMSADLRPCHGDWRHPKGLCPACRLFGTGSYKGRVRFGFASLE
NDPEWLIPGKNPGDPFHGGPVMLSLLERPRPTWSIPGSDNKFKVPGRKFY
VHHHAWKTIKDGNHPTTGKAIEQSPNNRTVEALAGGNSFSFEIAFENLKE
WELGLLIHSLQLEKGLAHKLGMAKSMGFGSVEIDVESVRLRKDWKQWRNG
NSEIPNWLGKGFAKLKEWFRDELDFIENLKKLLWFPEGDQAPRVCYPMLR
KKDDPNGNSGYEELKDGEFKKEDRQKKLTTPWTPWA

3D structure

• PDB: 7ync Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector.
• Chain: A
• Resolution: 3.14 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E13 R35 Q37 F39 H43 T59 R62 S63 K89 F90 D91 T92 K95 Q100 L101 R102 Q103 F150 N152 S154 K158 V172 L173 N174 R175 V176 K182 D185 F187 R227 F382 H383 G419 R444 S445 R448 R453 L467 G468 P471 V485 R501 T502 R503 I504 T510 L516 F517 S560 G561 S564 G641 R680 V682 S716 E719 H741 V742 I744 R746 K755 F757 H865 P908 T910 S948 E949 R967 R978 R1010 S1124 R1125 K1155 E1196 K1213 Y1214 C1215 Y1245 N1248 Q1250 R1294 G1349 T1350 Y1353 L1391 E1392 R1393 P1394 R1395 T1397 W1398 Y1415 A1488 K1489 Y1561 L1564 Y1576	E13 R35 Q37 F39 H43 T59 R62 S63 K89 F90 D91 T92 K95 Q100 L101 R102 Q103 F137 N139 S141 K145 V159 L160 N161 R162 V163 K169 D172 F174 R214 F336 H337 G373 R398 S399 R402 R407 L421 G422 P425 V439 R455 T456 R457 I458 T464 L470 F471 S514 G515 S518 G595 R634 V636 S670 E673 H695 V696 I698 R700 K709 F711 H819 P862 T864 S902 E903 R921 R932 R964 S1078 R1079 K1109 E1150 K1167 Y1168 C1169 Y1199 N1202 Q1204 R1248 G1284 T1285 Y1288 L1326 E1327 R1328 P1329 R1330 T1332 W1333 Y1350 A1423 K1424 Y1496 L1499 Y1511
BS02	rna	A	R283 D379 E513 G514 L516 F517 D654 E717 Y718 K754 K756 F757 A981 D982 H983 R1125 E1157 Q1250 L1390 N1441 L1564 R1565	R249 D333 E467 G468 L470 F471 D608 E671 Y672 K708 K710 F711 A935 D936 H937 R1079 E1111 Q1204 L1325 N1376 L1499 R1500

Gene Ontology

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:7ync, PDBe:7ync, PDBj:7ync
• PDBsum: 7ync
• PubMed: 36702942
• UniProt: A0A401FT36