Structure of PDB 7y9y Chain A

Receptor sequence

>7y9yA (length=1482) Species: 45657 (Desulfonema ishimotonii)

MTTTMKISIEFLEPFRMTKWQESTRRNKNNKEFVRGQAFARWHRNKKDNT
KGRPYITGTLLRSAVIRSAENLLTLSDGKISEKTCCPGKFDTEDKDRLLQ
LRQRSTLRWTDKNPCPDNAETYCPFCELLGRSFRIHFGNLSLPGKPDFDG
PKAIGSQRVLNRVDFKSGKAHDFFKAYEVDHTRFPRFEGEITIDNKVSAE
ARKLLCDSLKFTDRLCGALCVIRFDNLAEKTAEQIISILDDNKKTEYTRL
LADAIRSLRRSSKLVAGLPKDHDGKDDHYLWDIGVTIRQILTTSADTKEL
KNAGKWREFCEKLGEALYLKSKDSRSVSIGSVLKETVVCGELVAKTPFFF
GAIDEDAKQTALQVLLTPDNKYRLPRSAVRGILRRDLQTYFDSPCNAELG
GRPCMCKTCRIMRGITVMDARSEYNAPPEIRHRTRINPFTGTVAEGALFN
MEVAPEGIVFPFQLRYRGSEDGLPDALKTVLKWWAEGQAFMSGAASTGKG
RFRMENAKYETLDLSDENQRNDYLKNWGWRDEKGLEELKKRLNSGLPEPG
NYRDPKWHEINVSIEMASPFINGDPIRAAVDKRGTAVVTFVKYKAEGEEA
KPVCAYKAESFRGVIRSAVARIHCECLLCQIFGSEYEAGKIRFEDLVFES
DPEPVTFDHVAIDRFTGGAADKKKFDDSPLPGSPARPLMLKGSFWIRRDV
LEDEEYCKALGKALADVNNGLYPLGGKSAIGYGQVKSLGIKGDDKRISRL
MNVAVPEKPKTDAEVRIEAEKVYYPHYFVEPHKKVEREEKPCGHQKFHEG
RLTGKIRCKLITKTPLIVPDTSNDDFFYHKSYAFFRLHKQIMIPGSELRG
MVSSVYETVTNSCFRIFDETKRLSWRMDAQDFLPGRVGKHIQKFSETARV
PFYDKTQKHFDILDEQEIAGEKPVRMWVKRFIKRLSLVDPAWKRRKEGIA
TFIEQKNGSYYFNVVTNNGCTSFHLWHKPDNFDQEKLEGIQNGEKLDCWV
RDSRYQKAFQEIPENDPDGWECKEGYLHVVGPSKVEFSDKKGDVINNFQG
TLPSVPNDWKTIRTNDFKNRKRKNEPVFCCEDDKGNYYTMAKYCETFFFD
LKENEEYEIPEKARIKYKELLRVYNNNPQAVPESVFQSRVARENVEKLKS
GDLVYFKHNEKYVEDIVPVRISRTVDDRMIGKRMSADLRPCHGDWVEDGD
LSALNAYPEKRLLLRHPKGLCPACRLFGTGSYKGRVRFGFASLENDPEWL
IPGKNPGDPFHGGPVMLSLLERPRPTWSIPGSDNKFKVPGRKFYVHHHAW
KTIKDGNHPTTGKAIEQSPNNRTVEALAGGNSFSFEIAFENLKEWELGLL
IHSLQLEKGLAHKLGMAKSMGFGSVEIDVESVRLRKDWKQWRNGNSEIPN
WLGKGFAKLKEWFRDELDFIENLKKLLWFPEGDQAPRVCYPMLRKKDDPN
GNSGYEELKDGEFKKEDRQKKLTTPWTPWASS

3D structure

• PDB: 7y9y RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease.
• Chain: A
• Resolution: 2.77 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E13 R35 Q37 F39 H43 R53 Y55 T57 G58 T59 L60 R62 S63 R67 K89 F90 D91 T92 K95 Q100 L101 R102 Q103 R104 L129 R131 F146 H149 F150 N152 S154 V172 L173 N174 R175 V176 G181 K182 A183 D185 F186 F187 R227 G419 R444 S445 R448 G449 I450 R452 R453 L467 G468 G469 P471 R481 T484 V485 H500 R501 T502 R503 I504 L516 F517 G561 A562 A563 S564 G641 P643 E677 S678 R680 G681 V682 S716 E719 A720 D740 H741 V742 A743 I744 R746 G749 K756 F757 G808 P908 T910 S948 E949 R951 S956 R967 R978 R1010 R1125 S1154 K1155 R1193 N1195 E1196 K1213 Y1214 C1215 Y1245 N1248 Q1250 S1259 R1291 I1292 R1294 G1349 T1350 G1351 Y1353 K1354 L1391 E1392 R1393 P1394 W1398 Y1415 M1487 A1488 K1489 Y1561 L1564 Y1576 E1577	E13 R35 Q37 F39 H43 R53 Y55 T57 G58 T59 L60 R62 S63 R67 K89 F90 D91 T92 K95 Q100 L101 R102 Q103 R104 L129 R131 F133 H136 F137 N139 S141 V159 L160 N161 R162 V163 G168 K169 A170 D172 F173 F174 R214 G351 R376 S377 R380 G381 I382 R384 R385 L399 G400 G401 P403 R413 T416 V417 H432 R433 T434 R435 I436 L448 F449 G493 A494 A495 S496 G573 P575 E609 S610 R612 G613 V614 S634 E637 A638 D658 H659 V660 A661 I662 R664 G667 K674 F675 G726 P819 T821 S846 E847 R849 S854 R865 R876 R899 R1004 S1033 K1034 R1072 N1074 E1075 K1092 Y1093 C1094 Y1124 N1127 Q1129 S1138 R1170 I1171 R1173 G1228 T1229 G1230 Y1232 K1233 L1270 E1271 R1272 P1273 W1277 Y1294 M1366 A1367 K1368 Y1440 L1443 Y1455 E1456
BS02	rna	A	Y281 R283 H306 Y313 K364 A429 E513 G514 L516 F517 E717 D753 K754 K756 F757 A981 R1125 Q1127 K1155 E1157 F1158 S1159 D1160 P1249 Q1250 L1391 R1393 N1441 R1443 R1565	Y247 R249 H272 Y279 K322 A361 E445 G446 L448 F449 E635 D671 K672 K674 F675 A879 R1004 Q1006 K1034 E1036 F1037 S1038 D1039 P1128 Q1129 L1270 R1272 N1320 R1322 R1444
BS03	ZN	A	C86 C115 C123 C126	C86 C115 C123 C126
BS04	ZN	A	C463 C472 C477	C395 C404 C409
BS05	ZN	A	C706 C711	C624 C629
BS06	ZN	A	C965 C1312 C1342 C1345	C863 C1191 C1221 C1224

Gene Ontology

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:7y9y, PDBe:7y9y, PDBj:7y9y
• PDBsum: 7y9y
• PubMed: 36423304
• UniProt: A0A401FT36