Structure of PDB 7y8y Chain A

Receptor sequence

>7y8yA (length=1291) Species: 237368 (Candidatus Scalindua brodae)

DMNITVELTFFEPYRLVEWFDWDARKKSHSAMRGQAFAQWTWKGKGRTAG
KSFITGTLVRSAVIKAVEELLSLNNGKWEGVPCCNGSFQTDESKGKKPSF
LRKRHTLQWQANNKNICDKEEACPFCILLGRFDNAGKVHERNKDYDIHFS
NFDLDHKQEKNDLRLVDIASGRILNRVDFDTGKAKDYFRTWEADYETYGT
YTGRITLRNEHAKKLLLASLGFVDKLCGALCRIEVISEDHNDELRKQAEV
IVEAFKQNDKLEKIRILADAIRTLRLHGEGVIEKDELPDGKEERDKGHHL
WDIKVQGTALRTKLKELWQSNKDIGWRKFTEMLGSNLYLIYKKETGILGD
TEYYSKAHDSEGSDLFIPVTPPEGIETKEWIIVGRLKAATPFYFGVQQPS
DSIPGKESLVINEHTSFNILLDKENRYRIPRSALRGALRRDLRTAFGSGC
NVSLGGQILCNCKVCIEMRRITLKDSVSDFSEPPEIRYRIAKNPGTATVE
DGSLFDIEVGPEGLTFPFVLRYRGHKFPEQLSSVIRYWEENDGKNGMAWL
GGLDSTGKGRFALKDIKIFEWDLNQKINEYIKERGMRGKEKELLEMGESS
LPDGLIPYKFFEERECLFPYKENLKPQWSEVQYTIEVGSPLLTADTISAL
TEPGNRDAIAYKKRVYNDGNNAIEPEPRFAVKSETHRGIFRTAVGRRTGD
LGKEDHEDCTCDMCIIFGNEHESSKIRFEDLELINGNEFEKLEKHIDHVA
IDRFTGGALDKAKFDTYPLAGSPKKPLKLKGRFWIKKGFSGDHKLLITTA
LSDIRDGLYPLGSKGGVGYGWVAGISIDDNVPDDFKEMINKTEMPLPEEV
EESNNGPINNDYVHPGHQSPKQDHKNKNIYYPHYFLDSGSKVYREKDIIT
HEEFTEELLSGKINCKLETLTPLIIPDTSDENGLKLQGNKPGHKNYKFFN
INGELMIPGSELRGMLRTHFEALTKSCFAIFGEDSTLSWRCASKTLGGKL
DKALHPCTGLSDGLCPGCHLFGTTDYKGRVKFGFAKYENGPEWLITRGNN
PERSLTLGVLESPRPAFSIPDDESEIPGRKFYLHHNGWRIIRQKQLEIRE
TVQPERNVTTEVMDKGNVFSFDVRFENLREWELGLLLQSLDPGKNIAHKL
GKGKPYGFGSVKIKIDSLHTFKINSNNDKIKRVPQSDIREYINKGYQKLI
EWSGNNSIQKGNVLPQWHVIPHIDKLYKLLWVPFLNDSKLEPDVRYPVLN
EESKGYIEGSDYTYKKLGDKDNLPYKTRVKGLTTPWSPWNP

3D structure

• PDB: 7y8y Structure of Cas7-11-crRNA-tgRNA in complex with TPR-CHAT
• Chain: A
• Resolution: 3.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	W23 R37 T45 T61 L62 R64 S91 F92 Q93 T94 P102 F104 L105 R106 R108 G134 G140 K141 H143 Y149 H152 N155 D157 I177 L178 N179 V181 G186 D190 F192 K229 S391 S473 R476 R481 L495 G497 L514 R530 I531 A532 K533 T539 L545 F546 G593 S596 A685 T726 R728 R732 N760 H789 V790 A791 I792 R794 K804 F805 S854 P967 T969 E1002 R1008 R1031 T1422 L1459 E1460 P1462 R1463 F1466 K1479 Y1481 K1551 G1552 K1553 Y1645 Y1663	W19 R33 T41 T57 L58 R60 S87 F88 Q89 T90 P98 F100 L101 R102 R104 G130 G136 K137 H139 Y145 H148 N151 D153 I173 L174 N175 V177 G182 D186 F188 K225 S355 S432 R435 R440 L454 G456 L473 R489 I490 A491 K492 T498 L504 F505 G552 S555 A644 T685 R687 R691 N719 H748 V749 A750 I751 R753 K763 F764 S813 P926 T928 E961 R967 R990 T1023 L1060 E1061 P1063 R1064 F1067 K1080 Y1082 K1152 G1153 K1154 Y1246 Y1264
BS02	rna	A	K187 R294 E321 H328 Y370 L384 G385 T456 E541 G543 L545 E748 E761 H762 K802 F805 T1423 E1460 R1505	K183 R265 E292 H299 Y341 L348 G349 T415 E500 G502 L504 E707 E720 H721 K761 F764 T1024 E1061 R1106
BS03	ZN	A	C491 C501 C503 C506	C450 C460 C462 C465
BS04	ZN	A	C1018 C1417	C977 C1018
BS05	ZN	A	C88 C121 C130	C84 C117 C126
BS06	ZN	A	H747 C750 C752 C755	H706 C709 C711 C714

Gene Ontology

Molecular Function

• GO:0003723 RNA binding

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:7y8y, PDBe:7y8y, PDBj:7y8y
• PDBsum: 7y8y
• PubMed: 36280712
• UniProt: A0A0B0EGF3