Structure of PDB 7y82 Chain A

Receptor sequence

>7y82A (length=1341) Species: 237368 (Candidatus Scalindua brodae)

MNITVELTFFEPYRLVEWFDWDARKKSHSAMRGQAFAQWTWKGKGRTAGK
SFITGTLVRSAVIKAVEELLSLNNGKWEGVPCCNGSFQTDESKGKKPSFL
RKRHTLQWQANNKNICDKEEACPFCILLGRFDNAGKVHERNKDYDIHFSN
FDLDHDLRLVDIASGRILNRVDFDTGKAKDYFRTWEADYETYGTYTGRIT
LRNEHAKKLLLASLGFVDKLCGALCRIEVIDHNDELRKQAEVIVEAFKQN
DKLEKIRILADAIRTLRLHGEGVIEKDELPDGKEERDKGHHLWDIKVQGT
ALRTKLKELWQSNKDIGWRKFTEMLGSNLYLIYKKETTEYYSSDLFIPVT
PPEGIETKEWIIVGRLKAATPFYFGVQQPSDSIPGKEVINEHTSFNILLD
KENRYRIPRSALRGALRRDLRTAFGSGCNVSLGGQILCNCKVCIEMRRIT
LKDSVSDFSEPPEIRYRIAKNPGTATVEDGSLFDIEVGPEGLTFPFVLRY
RGHKFPEQLSSVIRYWEENDGKNGMAWLGGLDSTGKGRFALKDIKIFEWD
LNQKINEYIKERGMRGKEKELLEMGESSLPDGLIPYKFFEERECLFPYKE
NLKPQWSEVQYTIEVGSPLLTADTISALTEPGNRDAIAYKKRVYNDGNNA
IEPEPRFAVKSETHRGIFRTAVGRRTGDLGKEDHEDCTCDMCIIFGNEHE
SSKIRFEDLELINGNEFEKLEKHIDHVAIDRFTGGALDKAKFDTYPLAGS
PKKPLKLKGRFWIKKGFSGDHKLLITTALSDIRDGLYPLGSKGGVGYGWV
AGISIDDNVINNDYVHPGHQSPKQDHKNKNIYYPHYFLDSGSKVYREKDI
ITHEEFTEELLSGKINCKLETLTPLIIPDTSDENGLKLQGNKPGHKNYKF
FNINGELMIPGSELRGMLRTHFEALTKSCFAIFGEDSTLSWRMNADEKDY
VRDKNRVFLNAANNNRQYLNNIKKSNHDLYLQYLKGEKKIRFGYPVDDQI
KKNYEDILDSYDGIKDQEVAERFDTFTRGSKLKVGDLVYFHIIDSLIPVR
ISRKCASKTLGGKLDKALHPCTGLSDGLCPGCHLFGTTDYKGRVKFGFAK
YENGPEWLITRGNNPERSLTLGVLESPRPAFSIPDDESEIPGRKFYLHHN
GWRIIRQKQLEIRETVQPERNVTTEVMDKGNVFSFDVRFENLREWELGLL
LQSLDPGKNIAHKLGKGKPYGFGSVKIKIDSLHTFKIKRVPQSDIREYIN
KGYQKLIEWSGLPQWHVIPHIDKLYKLLWVPFLNDSKLEPDVRYPVLNEE
SKGYIEGSDYTYKKLGDKDNLPYKTRVKGLTTPWSPWNPFQ

3D structure

• PDB: 7y82 Structural basis for the non-self RNA-activated protease activity of the type III-E CRISPR nuclease-protease Craspase
• Chain: A
• Resolution: 2.83 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E16 R19 W23 R37 Q39 A40 F41 T45 K55 F57 G60 T61 L62 R64 S65 S91 F92 Q93 T94 K101 P102 S103 F104 L105 R106 K107 R108 L133 G134 R135 G140 K141 H143 Y149 I151 H152 F153 S154 N155 D157 I177 L178 N179 R180 V181 G186 A188 D190 Y191 F192 R208 K229 G232 Y389 S391 G431 R472 S473 A474 R476 G477 R481 L495 G497 L500 T513 L514 Y529 R530 I531 A532 K533 T539 V540 L545 F546 G592 G593 L594 S596 T684 A685 K723 E725 T726 R728 G729 I730 R732 T733 G759 N760 S765 D788 H789 V790 A791 I792 R794 G797 K804 F805 S854 K855 Y922 H924 P967 T969 S1001 E1002 R1004 G1005 R1008 I1021 R1031 F1183 Y1341 D1342 T1355 V1384 R1385 I1386 R1388 G1421 T1422 T1423 Y1425 K1426 L1459 E1460 S1461 P1462 R1463 A1465 F1466 K1479 Y1481 G1550 K1551 G1552 K1553 P1554 Y1645 L1648 Y1663	E11 R14 W18 R32 Q34 A35 F36 T40 K50 F52 G55 T56 L57 R59 S60 S86 F87 Q88 T89 K96 P97 S98 F99 L100 R101 K102 R103 L128 G129 R130 G135 K136 H138 Y144 I146 H147 F148 S149 N150 D152 I167 L168 N169 R170 V171 G176 A178 D180 Y181 F182 R198 K219 G222 Y340 S342 G375 R409 S410 A411 R413 G414 R418 L432 G434 L437 T450 L451 Y466 R467 I468 A469 K470 T476 V477 L482 F483 G529 G530 L531 S533 T621 A622 K660 E662 T663 R665 G666 I667 R669 T670 G696 N697 S702 D725 H726 V727 A728 I729 R731 G734 K741 F742 S791 K792 Y833 H835 P878 T880 S912 E913 R915 G916 R919 I932 R942 F958 Y1011 D1012 T1025 V1049 R1050 I1051 R1053 G1086 T1087 T1088 Y1090 K1091 L1124 E1125 S1126 P1127 R1128 A1130 F1131 K1144 Y1146 G1215 K1216 G1217 K1218 P1219 Y1294 L1297 Y1312
BS02	rna	A	A188 K189 K292 R294 K320 R323 K325 H328 Y367 K371 T387 S457 F458 G543 L545 F546 D698 E761 D801 K802 A803 K804 F805 A1034 K1345 E1460 S1461 L1648 N1649 E1651	A178 K179 K255 R257 K283 R286 K288 H291 Y330 K334 T338 S394 F395 G480 L482 F483 D635 E698 D738 K739 A740 K741 F742 A945 K1015 E1125 S1126 L1297 N1298 E1300
BS03	ZN	A	H747 C750 C752 C755	H684 C687 C689 C692
BS04	ZN	A	C1018 C1414 C1417	C929 C1079 C1082
BS05	MG	A	G134 D137 A139	G129 D132 A134
BS06	ZN	A	C88 C121 C127	C83 C116 C122

Gene Ontology

Molecular Function

• GO:0003723 RNA binding

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:7y82, PDBe:7y82, PDBj:7y82
• PDBsum: 7y82
• PubMed: 36477448
• UniProt: A0A0B0EGF3