Structure of PDB 7y81 Chain A

Receptor sequence

>7y81A (length=1223) Species: 237368 (Candidatus Scalindua brodae)

MNITVELTFFEPYRLVEWFDWDARKKSHSAMRGQAFAQWTWKGKGRTAGK
SFITGTLVRSAVIKAVEELLSLNNGKWEGVPCCNGSFQTDESKGKKPSFL
RKRHTLQWQANNKNICDKEEACPFCILLGRFDNAGKVHERNKDYDIHFSN
FDLDHDLRLVDIASGRILNRVDFDTGKAKDYFRTWEADYETYGTYTGRIT
LRNEHAKKLLLASLGFVDKLCGALCRIEVIDHNDELRKQAEVIVEAFKQN
DKLEKIRILADAIRTLRLHGEGVIEKDELPDGKEERDKGHHLWDIKVQGT
ALRTKLKELWQSNKDIGWRKFTEMLGSNLYLIYKKETTEYYSSDLFIPVT
PPEGIETKEWIIVGRLKAATPFYFGVQQPSDSIPGKEVINEHTSFNILLD
KENRYRIPRSALRGALRRDLRTAFGSGCNVSLGGQILCNCKVCIEMRRIT
LKDSVSDFSEPPEIRYRIAKNPGTATVEDGSLFDIEVGPEGLTFPFVLRY
RGHKFPEQLSSVIRYWEENDGKNGMAWLGGLDSTGKGRFALKDIKIFEWD
LNQKINEYIKERGMRGKEKELLEMGESSLPDGLIPYKFFEERECLFPYKE
NLKPQWSEVQYTIEVGSPLLTADTISALTEPGNRDAIAYKKRVYNDGNNA
IEPEPRFAVKSETHRGIFRTAVGRRTGDLGKEDHEDCTCDMCIIFGNEHE
SSKIRFEDLELINGNEFEKLEKHIDHVAIDRFTGGALDKAKFDTYPLAGS
PKKPLKLKGRFWIKKGFSGDHKLLITTALSDIRDGLYPLGSKGGVGYGWV
AGISIDDNVINNDYVHPGHQSPKQDHKNKNIYYPHYFLDSGSKVYREKDI
ITHEEFTEELLSGKINCKLETLTPLIIPDTSDENGLKLQGNKPGHKNYKF
FNINGELMIPGSELRGMLRTHFEALTKSCFAIFGEDSTLSASKTLGGKLD
KALHPCTGLSDGLCPGCHLFGTTDYKGRVKFGFAKYENGPEWLITRGNNP
ERSLTLGVLESPRPAFSIPDDESEIPGRKFYLHHNGWRIIRQKQLEIRET
VQPERNVTTEVMDKGNVFSFDVRFENLREWELGLLLQSLDPGKNIAHKLG
KGKPYGFGSVKIKIDSLHTFKIKRVPQSDIREYINKGYQKLIEWSGLPQW
HVIPHIDKLYKLLWVPFLNDSKLEPDVRYPVLNEESKGYIEGSDYTYKKL
GDKDNLPYKTRVKGLTTPWSPWN

3D structure

• PDB: 7y81 Structural basis for the non-self RNA-activated protease activity of the type III-E CRISPR nuclease-protease Craspase
• Chain: A
• Resolution: 2.54 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E16 R19 W23 R37 Q39 A40 F41 T45 K47 K55 F57 G60 T61 L62 R64 S65 S91 F92 Q93 T94 K98 K101 P102 S103 F104 L105 R106 K107 R108 L133 G134 R135 G140 K141 H143 Y149 H152 F153 N155 D157 R176 I177 L178 N179 R180 V181 G186 K187 A188 D190 Y191 F192 R208 K229 G232 Y389 S391 D400 G431 R472 S473 A474 R476 G477 R481 S494 L495 G496 L500 R510 T513 L514 Y529 R530 I531 A532 K533 T539 L545 F546 G592 G593 L594 S596 T684 A685 K723 E725 T726 R728 G729 I730 R732 T733 N760 E763 S764 S765 D788 H789 V790 A791 I792 R794 G797 K804 F805 G853 S854 Y922 P967 T969 S1001 E1002 R1004 G1005 R1008 T1009 I1021 F1420 G1421 T1422 T1423 Y1425 K1426 V1458 L1459 E1460 P1462 R1463 A1465 F1466 K1479 Y1481 G1550 K1551 G1552 K1553 P1554 Y1645 L1648 Y1663	E11 R14 W18 R32 Q34 A35 F36 T40 K42 K50 F52 G55 T56 L57 R59 S60 S86 F87 Q88 T89 K93 K96 P97 S98 F99 L100 R101 K102 R103 L128 G129 R130 G135 K136 H138 Y144 H147 F148 N150 D152 R166 I167 L168 N169 R170 V171 G176 K177 A178 D180 Y181 F182 R198 K219 G222 Y340 S342 D344 G375 R409 S410 A411 R413 G414 R418 S431 L432 G433 L437 R447 T450 L451 Y466 R467 I468 A469 K470 T476 L482 F483 G529 G530 L531 S533 T621 A622 K660 E662 T663 R665 G666 I667 R669 T670 N697 E700 S701 S702 D725 H726 V727 A728 I729 R731 G734 K741 F742 G790 S791 Y833 P878 T880 S912 E913 R915 G916 R919 T920 I932 F970 G971 T972 T973 Y975 K976 V1008 L1009 E1010 P1012 R1013 A1015 F1016 K1029 Y1031 G1100 K1101 G1102 K1103 P1104 Y1179 L1182 Y1197
BS02	rna	A	K187 K292 R294 K320 R323 H328 Y367 K371 E454 S457 F458 D542 G543 L545 F546 D698 E761 H762 D801 K802 A803 K804 F805 T1423 L1459 E1460 S1461 R1505 L1648	K177 K255 R257 K283 R286 H291 Y330 K334 E391 S394 F395 D479 G480 L482 F483 D635 E698 H699 D738 K739 A740 K741 F742 T973 L1009 E1010 S1011 R1055 L1182
BS03	ZN	A	H747 C750 C752 C755	H684 C687 C689 C692
BS04	ZN	A	C491 C501 C503 C506	C428 C438 C440 C443
BS05	ZN	A	C1018 C1406 C1414 C1417	C929 C956 C964 C967
BS06	MG	A	G134 D137 A139	G129 D132 A134
BS07	ZN	A	C88 C121 C127 C130	C83 C116 C122 C125

Gene Ontology

Molecular Function

• GO:0003723 RNA binding

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:7y81, PDBe:7y81, PDBj:7y81
• PDBsum: 7y81
• PubMed: 36477448
• UniProt: A0A0B0EGF3