Structure of PDB 7y0r Chain A

Receptor sequence

>7y0rA (length=452) Species: 1348623 (Priestia megaterium NBRC 15308 = ATCC 14581)

KEMPQPKTFGELKNLPLLNTDKPVQALMKIADELGEIFKFEAPGRVTRYL
SSQRLIKEACDESRFDKNLSQALKFSRDFAGDGLLTSWTHEKNWKKAHNI
LLPSFSQQAMKGYHAMMVDIAVQLVQKWERLNADEHIEVPEDMTRLTLDT
IGLCGFNYRFNSFYRDQPHPFITSMVRALDEVMNKLQRANPDDPAYDENK
RQFQEDIKVMNDLVDKIIADRKASEQSDDLLTHMLNGKDPETGEPLDDEN
IRYQIITFLIAGHETTSGLLSFALYFLVKNPHVLQKAAEEAARVLVDPVP
SYKQVKQLKYVGMVLNEALRLWPTAPAFSLYAKEDTVLGGEYPLEKGDEL
MVLIPQLHRDKTIWGDDVEEFRPERFENPSAIPQHAFKPFGNGQRACIGQ
QFALHEATLVLGMMLKHFDFEDHTNYELDIKETLTLKPEGFVVKAKSKKI
PL

3D structure

• PDB: 7y0r Crystal structure of the P450 BM3 heme domain mutant F87L/V78S/A184V in complex with N-imidazolyl-hexanoyl-L-phenylalanine, p-toluidine and hydroxylamine
• Chain: A
• Resolution: 2.09 Å

Enzymatic activity

• Enzyme Commision number: 1.14.14.1: unspecific monooxygenase.
  1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	K69 L86 L87 W96 F261 A264 G265 T268 T269 L272 A328 F331 P392 F393 R398 C400 I401 G402 A406	K67 L84 L85 W94 F258 A261 G262 T265 T266 L269 A325 F328 P389 F390 R395 C397 I398 G399 A403
BS02	HOA	A	A264 T268	A261 T265
BS03	IC6	A	P25 Y51 M354	P23 Y49 M351
BS04	IC6	A	L20 R47 Y51 S72 Q73 A74	L18 R45 Y49 S70 Q71 A72

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0020037 heme binding

External links

• PDB: RCSB:7y0r, PDBe:7y0r, PDBj:7y0r
• PDBsum: 7y0r
• UniProt: P14779|CPXB_PRIM2 Bifunctional cytochrome P450/NADPH--P450 reductase (Gene Name=cyp102A1)