Structure of PDB 7x1o Chain A

Receptor sequence

>7x1oA (length=467) Species: 9606 (Homo sapiens)

LEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFD
AEIKKLGVENCYFPMFVSQSALEKEPEVAWVTRSGKTELAEPIAIRPTSE
TVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGH
SAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTT
TIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSW
GLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCSEEDKEALIAKCND
YRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQF
VAVRRDTGEKLTVAEETKLQAILEDIQVTLFTRASEDLKTHMVVANTMED
FQKILDSGKIVQIPFCGEIDCEDWIKKTTAMGAKSLCIPFKPLCELGAKC
VCGKNPAKYYTLFGRSY

3D structure

• PDB: 7x1o Crystal structure of Homo sapiens Prolyl-tRNA synthetase (HsPRS) with double inhibitors (HF and L95)
• Chain: A
• Resolution: 2.04 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.15: proline--tRNA ligase.
  6.1.1.17: glutamate--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HFG	A	E1100 P1120 T1121 E1123 R1152 W1169 F1216 H1242 S1272 W1273 G1274	E77 P97 T98 E100 R129 W146 F193 H219 S249 W250 G251
BS02	JE6	A	R1152 E1154 R1163 T1164 F1167 Q1237 G1239 G1274 T1276 R1278	R129 E131 R140 T141 F144 Q214 G216 G251 T253 R255
BS03	ZN	A	C1448 C1453 C1495 C1497	C416 C421 C450 C452

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004827 proline-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006433 prolyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7x1o, PDBe:7x1o, PDBj:7x1o
• PDBsum: 7x1o
• UniProt: P07814|SYEP_HUMAN Bifunctional glutamate/proline--tRNA ligase (Gene Name=EPRS1)