Structure of PDB 7x09 Chain A

Receptor sequence

>7x09A (length=468) Species: 9606 (Homo sapiens)

LEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFD
AEIKKLGVENCYFPMFVSQSALEKPEVAWVTRSGKTELAEPIAIRPTSET
VMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHS
AFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTT
IEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWG
LTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCSEEDKEALIAKCNDY
RRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFV
AVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTM
EDFQKILDSGKIVQIPFCGEIDCEDWIKKTTASMGAKSLCIPFKPLCELK
CVCGKNPAKYYTLFGRSY

3D structure

• PDB: 7x09 Crystal structure of Homo sapiens Prolyl-tRNA synthetase (HsPRS) with double inhibitors (HF and L95)
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.15: proline--tRNA ligase.
  6.1.1.17: glutamate--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HFG	A	E1100 V1101 P1120 T1121 E1123 R1152 W1169 F1216 H1242 S1272 G1274	E76 V77 P96 T97 E99 R128 W145 F192 H218 S248 G250
BS02	JE6	A	E1154 K1156 R1163 T1164 F1167 Q1237 G1239 G1274 T1276 R1278	E130 K132 R139 T140 F143 Q213 G215 G250 T252 R254
BS03	ZN	A	C1448 C1453 C1495 C1497	C418 C423 C451 C453

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004827 proline-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006433 prolyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7x09, PDBe:7x09, PDBj:7x09
• PDBsum: 7x09
• UniProt: P07814|SYEP_HUMAN Bifunctional glutamate/proline--tRNA ligase (Gene Name=EPRS1)