Structure of PDB 7wtb Chain A

Receptor sequence

>7wtbA (length=1146) Species: 9606 (Homo sapiens)

EYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADE
AYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQ
DAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHE
FSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGA
LFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAH
LDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVE
HTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTED
PARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAH
GKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFID
ENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAV
PIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRV
RTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRE
LIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNY
LPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVR
AGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAA
MLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVF
DYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMG
LGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAE
AQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASL
PPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDS
LNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNG
QLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVA
KGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

3D structure

• PDB: 7wtb Mechanistic insight into allosteric activation of human pyruvate carboxylase by acetyl-CoA.
• Chain: A
• Resolution: 3.7 Å

Enzymatic activity

• Enzyme Commision number: 6.4.1.1: pyruvate carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	A	G199 M204 F238 I239 H271 E324	G167 M172 F206 I207 H239 E292
BS02	ACO	A	E449 R451 R453 Q494 N495 R496 K1056 L1058	E417 R419 R421 Q462 N463 R464 K1024 L1026
BS03	ACO	A	F53 T57 R77 Q78 K79 A80	F21 T25 R45 Q46 K47 A48
BS04	BTI	A	Y651 Q870 T908 S911 K912	Y619 Q838 T876 S879 K880

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004736 pyruvate carboxylase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0009374 biotin binding
• GO:0016874 ligase activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006094 gluconeogenesis
• GO:0006629 lipid metabolic process
• GO:0006734 NADH metabolic process
• GO:0006739 NADP metabolic process
• GO:0010629 negative regulation of gene expression
• GO:0019074 viral RNA genome packaging
• GO:0019076 viral release from host cell
• GO:0044794 positive regulation by host of viral process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0005829 cytosol

External links

• PDB: RCSB:7wtb, PDBe:7wtb, PDBj:7wtb
• PDBsum: 7wtb
• PubMed: 36283412
• UniProt: P11498|PYC_HUMAN Pyruvate carboxylase, mitochondrial (Gene Name=PC)