Structure of PDB 7wjd Chain A

Receptor sequence

>7wjdA (length=729) Species: 416870 (Lactococcus cremoris subsp. cremoris MG1363)

NNNIIKFDKARFTVLTEHLIRIEYSETGEFEERMTQMVQNREFSEVNFDI
IEKEETIEIITSTVHLYYNGGEFTNASLFADVKFNFSVYSNRWYFGEKSD
GNLKGTTRTLDMIDGECPLEDGIMSKNGFAVLADKGKVLTEVGDIAGNSV
STIDLYLFAYGRDYRQALKDFYQLTGNTPKLPRFALGNWWSRYYDYSDKS
YLALMDKFTDKKVPLSVSVIDMDWHKVSEVPSRFGSGWTGYSWNKKLFPN
PENFIDELHQRKLKVTLNDHPADGIRAFEDPYPQVAQTLDLNTELEEAAK
FDFDNLKFRKAYFEEVHGPLEKEGVDFWWIDWQQGAISKSGVDPLWLLNH
YQYQNAQKKHKNNIILSRYAGPGSHRYPLGFSGASVISWASLDFQPYFTS
TASNIGYTWWSHDIGGHMQGYKDAELSLRWLQFGVFSPINRLHSSKSEFT
SKEPWHFDAVIEQSMIDFLQLRHQLIPYLYSANLITASEGRALVEPLYYE
YPMEEEAYQHRNQYLFGEQLMVAPITEKMNSLLQMGSVEVWFPEGTWYDF
FSGQPYDGKVSLKVYREITEMPVFAKAGAIIPLDKNPLKKEEIPSEIIWK
IFPGADGEYLLLEEDNETKAEFVNGIFTVTSKKESSRKHTIIYGEHEIVS
AKRGEFSIDLNGKEENFDWNFSTALFRRLDIAEISYEQKDEILQQLSLIE
EHEKQVAFIKTNENQELQNSLFELLYSGK

3D structure

• PDB: 7wjd Structural basis of the strict specificity of a bacterial GH31 alpha-1,3-glucosidase for nigerooligosaccharides.
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	Y203 Q343 H427 M428 S455	Y193 Q333 H417 M418 S445
BS02	GLC	A	Y203 D231 M232 W248 N278 H280 D341 H427 H453	Y193 D221 M222 W238 N268 H270 D331 H417 H443
BS03	BGC	A	Y203 W248	Y193 W238

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016798 hydrolase activity, acting on glycosyl bonds

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:7wjd, PDBe:7wjd, PDBj:7wjd
• PDBsum: 7wjd
• PubMed: 35293315
• UniProt: A2RM80