Structure of PDB 7wfw Chain A

Receptor sequence

>7wfwA (length=998) Species: 9606 (Homo sapiens)

LKVIVGALIHSVKKLADVTILTIFCLSVFALVGLQLFKGNLKNKCVKNDP
DIYINKRGTSDPLLCGNGSDSGHCPDGYICLKTSDNPDFNYTSFDSFAWA
FLSLFRLMTQDSWERLYQQTLRTSGKIYMIFFVLVIFLGSFYLVNLILAV
VTMAYEEQNQATWVKLKTILFGLVTDPFAELTITLCIVVNTIFMAMEHHG
MSPTFEAMLQIGNIVFTIFFTAEMVFKIIAFDPYYYFQKKWNIFDCIIVT
VSLLELGVAKKGSLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALG
NLTIILAIIVFVFALVGKQLLGENYRNNRKNISAPHEDWPRWHMHDFFHS
FLIVFRILCGEWIENMWACMEVGQKSICLILFLTVMVLGNLVVLNLFIAL
LLNSFFADVGWQVRKTCYRIVEHSWFESFIIFMILLSSGSLAFEDYYLDQ
KPTVKALLEYTDRVFTFIFVFEMLLKWVAYGFKKYFTNAWCWLDFLIVNI
SLISLTAKILEYSEVAPIKALRTLRALRPLRALSRFEGMRVVVDALVGAI
PSIMNVLLVCLIFWLIFSIMGVNLFAGKFWRCINYTDGEFSLVPLSIVNN
KSDCKIQNSTGSFFWVNVKVNFDNVAMGYLALLQVATFKGWMDIMYAAVD
SREVNMQPKWEDNVYMYLYFVIFIIFGGFFTLNLFVGVIIDNFNQQKKKL
GGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKFQGFVFDIVTRQAF
DITIMVLICLNMITMMVETDDQSEEKTKILGKINQFFVAVFTGECVMKMF
ALRQYYFTNGWNVFDFIVVVLSIASLIFSAILKSLQSYFSPTLFRVIRLA
RIGRILRLIRAAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSS
FPHVRWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYC
DPNLPNSNGTRGDCGSPAVGIIFFTTYIIISFLIMVNMYIAVILENFN

3D structure

• PDB: 7wfw Structural basis for high-voltage activation and subtype-specific inhibition of human Na v 1.8.
• Chain: A
• Resolution: 3.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CLR	A	S769 W770 I1290	S281 W282 I562
BS02	LPE	A	P1605 M1716	P877 M988
BS03	LPE	A	I372 Y373	I127 Y128
BS04	CLR	A	I731 F732 L761	I243 F244 L273
BS05	LPE	A	N1216 W1218	N488 W490
BS06	CLR	A	A1217 W1220	A489 W492
BS07	LPE	A	A1170 F1171 F1615 F1619 F1647	A442 F443 F887 F891 F919
BS08	CLR	A	I1491 M1494	I763 M766
BS09	P5S	A	W344 F350 K863 S864 L867 L871	W99 F105 K375 S376 L379 L383
BS10	P5S	A	W1292 L1361 A1696 I1699 T1703 T1704 I1707	W564 L633 A968 I971 T975 T976 I979

Gene Ontology

Molecular Function

• GO:0005216 monoatomic ion channel activity
• GO:0005248 voltage-gated sodium channel activity
• GO:0005261 monoatomic cation channel activity
• GO:0005272 sodium channel activity
• GO:0044325 transmembrane transporter binding
• GO:0099508 voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential

Biological Process

• GO:0002027 regulation of heart rate
• GO:0006811 monoatomic ion transport
• GO:0006814 sodium ion transport
• GO:0007600 sensory perception
• GO:0034220 monoatomic ion transmembrane transport
• GO:0034765 regulation of monoatomic ion transmembrane transport
• GO:0035725 sodium ion transmembrane transport
• GO:0042475 odontogenesis of dentin-containing tooth
• GO:0055085 transmembrane transport
• GO:0055117 regulation of cardiac muscle contraction
• GO:0060371 regulation of atrial cardiac muscle cell membrane depolarization
• GO:0086002 cardiac muscle cell action potential involved in contraction
• GO:0086010 membrane depolarization during action potential
• GO:0086016 AV node cell action potential
• GO:0086043 bundle of His cell action potential
• GO:0099505 regulation of presynaptic membrane potential

Cellular Component

• GO:0001518 voltage-gated sodium channel complex
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0030424 axon
• GO:0034702 monoatomic ion channel complex
• GO:0042734 presynaptic membrane
• GO:0070062 extracellular exosome
• GO:0071439 clathrin complex
• GO:0098978 glutamatergic synapse

External links

• PDB: RCSB:7wfw, PDBe:7wfw, PDBj:7wfw
• PDBsum: 7wfw
• PubMed: 35858452
• UniProt: Q9Y5Y9|SCNAA_HUMAN Sodium channel protein type 10 subunit alpha (Gene Name=SCN10A)