Structure of PDB 7we4 Chain A

Receptor sequence

>7we4A (length=1122) Species: 9606 (Homo sapiens)

LIRRTAIKVSVHSWFSLFITVTILVNCVCMTRTDLPEKIEYVFTVIYTFE
ALIKILARGFCLNEFTYLRDPWNWLDFSVITLAYVGTAIDLRGISGLRTF
RVLRALKTVSVIPGLKVIVGALIHSVKKLADVTILTIFCLSVFALVGLQL
FKGNLKNKCVKNDMAYSSHRKPDIYINKRGTSDPLLCGNGSDSGHCPDGY
ICLKTSDNPDFNYTSFDSFAWAFLSLFRLMTQDSWERLYQQTLRTSGKIY
MIFFVLVIFLGSFYLVNLILAVVTMAYEEQNQATWVKLKTILFGLVTDPF
AELTITLCIVVNTIFMAMEHHGMSPTFEAMLQIGNIVFTIFFTAEMVFKI
IAFDPYYYFQKKWNIFDCIIVTVSLLELGVAKKGSLSVLRSFRLLRVFKL
AKSWPTLNTLIKIIGNSVGALGNLTIILAIIVFVFALVGKQLLGENYRNN
RKNISAPHEDWPRWHMHDFFHSFLIVFRILCGEWIENMWACMEVGQKSIC
LILFLTVMVLGNLVVLNLFIALLLNSFFADVGWQVRKTCYRIVEHSWFES
FIIFMILLSSGSLAFEDYYLDQKPTVKALLEYTDRVFTFIFVFEMLLKWV
AYGFKKYFTNAWCWLDFLIVNISLISLTAKILEYSEVAPIKALRTLRALR
PLRALSRFEGMRVVVDALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK
FWRCINYTDGEFSLVPLSIVNNKSDCKIQNSTGSFFWVNVKVNFDNVAMG
YLALLQVATFKGWMDIMYAAVDSREVNMQPKWEDNVYMYLYFVIFIIFGG
FFTLNLFVGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQK
PIPRPLNKFQGFVFDIVTRQAFDITIMVLICLNMITMMVETDDQSEEKTK
ILGKINQFFVAVFTGECVMKMFALRQYYFTNGWNVFDFIVVVLSIASLIF
SAILKSLQSYFSPTLFRVIRLARIGRILRLIRAAKGIRTLLFALMMSLPA
LFNIGLLLFLVMFIYSIFGMSSFPHVRWEAGIDDMFNFQTFANSMLCLFQ
ITTSAGWDGLLSPILNTGPPYCDPNLPNSNGTRGDCGSPAVGIIFFTTYI
IISFLIMVNMYIAVILENFNVA

3D structure

• PDB: 7we4 Structural basis for high-voltage activation and subtype-specific inhibition of human Na v 1.8.
• Chain: A
• Resolution: 2.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	95T	A	Q355 L1614 T1659 F1710 M1713	Q232 L1008 T1053 F1104 M1107
BS02	CLR	A	T679 L766 W770 I1290	T313 L400 W404 I684
BS03	LPE	A	Q1439 P1605	Q833 P999
BS04	LPE	A	V784 F843 I1402 I1403 F1404 F1407	V418 F477 I796 I797 F798 F801
BS05	LPE	A	F376 F1567 S1568	F253 F961 S962
BS06	LPE	A	A1170 F1171 F1615 L1616 F1619 F1647	A564 F565 F1009 L1010 F1013 F1041
BS07	LPE	A	I680 S690 T692	I314 S324 T326
BS08	CLR	A	S341 M1494 V1495	S218 M888 V889
BS09	P5S	A	A1354 P1695 A1696 I1699 I1707	A748 P1089 A1090 I1093 I1101
BS10	P5S	A	R1475 M1525	R869 M919
BS11	P5S	A	W344 F350 K863 L871	W221 F227 K497 L505
BS12	CLR	A	L393 T397 E401 L889 V1414 I1418 V1714 Y1717 I1718 I1721	L270 T274 E278 L523 V808 I812 V1108 Y1111 I1112 I1115

Gene Ontology

Molecular Function

• GO:0005216 monoatomic ion channel activity
• GO:0005248 voltage-gated sodium channel activity
• GO:0005261 monoatomic cation channel activity
• GO:0005272 sodium channel activity
• GO:0044325 transmembrane transporter binding
• GO:0099508 voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential

Biological Process

• GO:0002027 regulation of heart rate
• GO:0006811 monoatomic ion transport
• GO:0006814 sodium ion transport
• GO:0007600 sensory perception
• GO:0034220 monoatomic ion transmembrane transport
• GO:0034765 regulation of monoatomic ion transmembrane transport
• GO:0035725 sodium ion transmembrane transport
• GO:0042475 odontogenesis of dentin-containing tooth
• GO:0055085 transmembrane transport
• GO:0055117 regulation of cardiac muscle contraction
• GO:0060371 regulation of atrial cardiac muscle cell membrane depolarization
• GO:0086002 cardiac muscle cell action potential involved in contraction
• GO:0086010 membrane depolarization during action potential
• GO:0086016 AV node cell action potential
• GO:0086043 bundle of His cell action potential
• GO:0099505 regulation of presynaptic membrane potential

Cellular Component

• GO:0001518 voltage-gated sodium channel complex
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0030424 axon
• GO:0034702 monoatomic ion channel complex
• GO:0042734 presynaptic membrane
• GO:0070062 extracellular exosome
• GO:0071439 clathrin complex
• GO:0098978 glutamatergic synapse

External links

• PDB: RCSB:7we4, PDBe:7we4, PDBj:7we4
• PDBsum: 7we4
• PubMed: 35858452
• UniProt: Q9Y5Y9|SCNAA_HUMAN Sodium channel protein type 10 subunit alpha (Gene Name=SCN10A)