Structure of PDB 7wah Chain A

Receptor sequence

>7wahA (length=1473) Species: 45657 (Desulfonema ishimotonii)

MTTTMKISIEFLEPFRMTKWQESTRRNKNNKEFVRGQAFARWHRNKKDNT
KGRPYITGTLLRSAVIRSAENLLTLSDGKISEKTCCPGKFDTEDKDRLLQ
LRQRSTLRWTDKNPCPDNAETYCPFCELLGRSFRIHFGNLSLPGKPDFDG
PKAIGSQRVLNRVDFKSGKAHDFFKAYEVDHTRFPRFEGEITIDNKVSAE
ARKLLCDSLKFTDRLCGALCVIRFDNLAEKTAEQIISILDDNKKTEYTRL
LADAIRSLRRSSKLVAGLPKDHDGKDDHYLWDIGVTIRQILTTSADTKEL
KNAGKWREFCEKLGEALYLKSKSVLKETVVCGELVAKTPFFFGAIDEDAK
QTALQVLLTPDNKYRLPRSAVRGILRRDLQTYFDSPCNAELGGRPCMCKT
CRIMRGITVMDARSEYNAPPEIRHRTRINPFTGTVAEGALFNMEVAPEGI
VFPFQLRYRGSEDGLPDALKTVLKWWAEGQAFMSGAASTGKGRFRMENAK
YETLDLSDENQRNDYLKNWGWRDEKGLEELKKRLNSGLPEPGNYRDPKWH
EINVSIEMASPFINGDPIRAAVDKRGTAVVTFVKYKAEGEEAKPVCAYKA
ESFRGVIRSAVARIHMEDGVPLTELTHSDCECLLCQIFGSEYEAGKIRFE
DLVFESDPEPVTFDHVAIDRFTGGAAAKKKFDDSPLPGSPARPLMLKGSF
WIRRDVLEDEEYCKALGKALADVNNGLYPLGGKSAIGYGQVKSLGIKGDD
KRISRLMNAVPEKPKTDAEVRIEAEKVYYPHYFVEPHKKVEREEKPCGHQ
KFHEGRLTGKIRCKLITKTPLIVPDTSNDDFFRPYHKSYAFFRLHKQIMI
PGSELRGMVSSVYETVTNSCFRIFDETKRLSWRMDADQDFLPGRVTADGK
HIQKFSETARVPFYDKTQKHFDILDEQEIAGEKPVRMWVKRFIKRLSLVD
PAKHWKRRKEGIATFIEQKNGSYYFNVVTNNGCTSFHLWHKPDNFDQEKL
EGIQNGEKLDCWVRDSRYQKAFQEIPENDPDGWECKEGYLHVVGPSKVEF
SDKKGDVINNFQGTLPSVPNDWKTIRTNDFKNRKRKNEPVFCCEDDKGNY
YTMAKYCETFFFDLKENEEYEIPEKARIKYKELLRVYNNNPQAVPESVFQ
SRVARENVEKLKSGDLVYFKHNEKYVEDIVPVRISRTVDDRMIGKRMSAD
LRPCHGDWKGLCPACRLFGTGSYKGRVRFGFASLENDPEWLIPGKNPGDP
FHGGPVMLSLLERPRPTWSIPGSDNKFKVPGRKFYVHHHAWKTIKDGNHP
TTGKAIEQSPNNRTVEALAGGNSFSFEIAFENLKEWELGLLIHSLQLEKG
LAHKLGMAKSMGFGSVEIDVESVRLRKDWKQWRNGNSEIPNWLGKGFAKL
KEWFRDELDFIENLKKLLWFPEGDQAPRVCYPMLRKKDDPNGNSGYEELK
DGEFKKEDRQKKLTTPWTPWASS

3D structure

• PDB: 7wah Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex.
• Chain: A
• Resolution: 2.45 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E13 R35 Q37 F39 H43 R53 Y55 G58 T59 L60 R62 R67 K89 F90 D91 T92 K95 R97 Q100 L101 R102 Q103 R104 L129 H149 F150 N152 K158 V172 L173 N174 R175 V176 G181 D185 F186 F187 R227 G419 R444 S445 A446 R448 G449 I450 R453 L467 G468 G469 P471 R481 T484 V485 H500 R501 T502 R503 I504 T510 L516 F517 G561 A562 A563 S564 N640 G641 P643 K675 E677 S678 R680 G681 V682 R684 S716 E719 A720 D740 H741 V742 A743 I744 R746 G749 K756 F757 G808 K809 A811 P908 T910 S948 E949 R951 R967 R978 R1010 R1045 H1098 K1099 S1124 R1125 S1154 K1155 R1193 N1195 E1196 K1213 Y1214 C1215 Y1245 N1248 Q1250 V1290 R1291 I1292 R1294 G1349 T1350 G1351 Y1353 K1354 L1391 E1392 R1393 P1394 R1395 T1397 W1398 Y1415 R1443 G1486 A1488 K1489 Y1561 L1564 Y1576 E1577	E13 R35 Q37 F39 H43 R53 Y55 G58 T59 L60 R62 R67 K89 F90 D91 T92 K95 R97 Q100 L101 R102 Q103 R104 L129 H136 F137 N139 K145 V159 L160 N161 R162 V163 G168 D172 F173 F174 R214 G343 R368 S369 A370 R372 G373 I374 R377 L391 G392 G393 P395 R405 T408 V409 H424 R425 T426 R427 I428 T434 L440 F441 G485 A486 A487 S488 N564 G565 P567 K599 E601 S602 R604 G605 V606 R608 S640 E643 A644 D664 H665 V666 A667 I668 R670 G673 K680 F681 G732 K733 A735 P824 T826 S853 E854 R856 R872 R883 R910 R945 H990 K991 S1016 R1017 S1046 K1047 R1085 N1087 E1088 K1105 Y1106 C1107 Y1137 N1140 Q1142 V1182 R1183 I1184 R1186 G1219 T1220 G1221 Y1223 K1224 L1261 E1262 R1263 P1264 R1265 T1267 W1268 Y1285 R1313 G1356 A1358 K1359 Y1431 L1434 Y1446 E1447
BS02	rna	A	Y281 R283 H306 Y313 G514 L516 F517 E717 A753 K754 K755 K756 F757 A981 R1125 E1157 S1159 D1160 Q1250 R1393 N1441 R1443 R1565	Y247 R249 H272 Y279 G438 L440 F441 E641 A677 K678 K679 K680 F681 A886 R1017 E1049 S1051 D1052 Q1142 R1263 N1311 R1313 R1435
BS03	ZN	A	C86 C115 C123 C126	C86 C115 C123 C126
BS04	ZN	A	C472 C474 C477	C396 C398 C401
BS05	ZN	A	H703 C711	H627 C635
BS06	ZN	A	C965 C1312 C1345	C870 C1204 C1215

Gene Ontology

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:7wah, PDBe:7wah, PDBj:7wah
• PDBsum: 7wah
• PubMed: 35643083
• UniProt: A0A401FT36