Structure of PDB 7wae Chain A

Receptor sequence

>7waeA (length=877) Species: 203124 (Trichodesmium erythraeum IMS101)

MKILKLQTLRGPNYWSIHRHKLVVMRLDLEDLYEKYTSDIPGFYKGLTEV
LPSLVEHLCSPGVKGGFLTRVEKGTLIGHVIEHVAIELQELAGMPVGFGR
TRETSTTGVFQVVIEYENEQAGRYAARAAVRLCQSIVDTGTYPATELQQD
LEDLKELKNQASLGPSTEAIVKEAEARGIPWTQLGARFMIQFGYGVNQKK
IQATLSNQTGILGVELACDKEGTKRILKDAGVPVPRGTVARYFDELQDAI
EYVGGYPIVIKPLDGNHGRGITIDVKNWQEAEEAYDLARKASKTKTVIVE
RYYTGKDHRVLVVNGKVVAVAERVPAHVVGNGKSTIAELIEETNRDPQRG
DGHDNILTRITVDKSALDILGKQGYSIDSIPLKGKKCFLRATANLSTGGI
AVDRTDEIHPENVWLLSRVAKIIGLDIAGIDVVTEDISQPLREVEGVIVE
VNAAPGFRMHVAPSRGLARNVAGAVMDMLFPGSKNGRIPILSVTGTNGKT
TTTRLLAHIIKQTGKVVGYTTTDGTYIGEYLAETGDNTGPQSAHLILSDP
TVEVAVLETARGGILRSGLGFSSCEVGIVLNVTADHLGIGDIDTIEQLAK
LKSVVAESVMPKGYAVLNAEDPLVAAMADRVKGQVAYFSMDPNNELLLRH
TEAGGLAAIYENGYISILKGDWTLRIEKAVNVPITMAGKAPFMIANALAA
CLAVFTQGVKIEHIRKGLSTFVASVDQTPGRMNMFNMGSYHALVDYAHNP
ASYEALGGFVRNWPGKRIGVVGGPGDRRDEDFVSLGELAADIFDEIIIKE
DDDTRGRPRGNAAELICQGVKQFLNGIKNSESKATYESILDETAAINTAL
DRAPIDGLVVILPESVNRAISLIEGRH

3D structure

• PDB: 7wae Structural bases for aspartate recognition and polymerization efficiency of cyanobacterial cyanophycin synthetase.
• Chain: A
• Resolution: 2.64 Å

Enzymatic activity

• Enzyme Commision number: 6.3.2.29: cyanophycin synthase (L-aspartate-adding).
  6.3.2.30: cyanophycin synthase (L-arginine-adding).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	S166 A454 P455 G456	S166 A454 P455 G456
BS02	MG	A	T500 T522 E558	T500 T522 E558
BS03	AGS	A	K261 G268 I271 E300 Y303 D307 E450	K261 G268 I271 E300 Y303 D307 E450
BS04	AGS	A	N497 G498 K499 T500 E558 F692 N696 R731 D745 Y746 H748 S752 A755	N497 G498 K499 T500 E558 F692 N696 R731 D745 Y746 H748 S752 A755

Gene Ontology

Molecular Function

• GO:0004326 tetrahydrofolylpolyglutamate synthase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0016881 acid-amino acid ligase activity
• GO:0046872 metal ion binding
• GO:0071160 cyanophycin synthetase activity (L-aspartate-adding)
• GO:0071161 cyanophycin synthetase activity (L-arginine-adding)

Biological Process

• GO:0009058 biosynthetic process
• GO:0009059 macromolecule biosynthetic process
• GO:0009396 folic acid-containing compound biosynthetic process
• GO:0046901 tetrahydrofolylpolyglutamate biosynthetic process

External links

• PDB: RCSB:7wae, PDBe:7wae, PDBj:7wae
• PDBsum: 7wae
• PubMed: 36042318
• UniProt: Q113V7