Structure of PDB 7w9s Chain A

Receptor sequence

>7w9sA (length=463) Species: 39054 (Enterovirus A71)

GEIQWVKPNKETGRLNINGPTRTKLEPSVFHDVFEGNKEPAVLHSKDPRL
EVDFEQALFSKYVGNTLYEPDEYIKEAALHYANQLKQLDIDTSQMSMEEA
CYGTENLEAIDLHTSAGYPYSALGIKKRDILDSTTRDVSKMKFYMDKYGL
DLPYSTYVKDELRSIDKIKKGKSRLIEASSLNDSVYLRMTFGHLYETFHA
NPGTVTGSAVGCNPDTFWSKLPILLPGSLFAFDYSGYDASLSPVWFRALE
LVLREIGYSEEAVSLVEGINHTHHVYRNKTYCVLGGMPSGMSGTSIFNSM
INNIIIRALLIKTFKGIDLDELNMVAYGDDVLASYPFPIDCLELARTGKE
YGLTMTPADKSPCFNEVNWDNATFLKRGFLPDEQFPFLIHPTMPMKEIHE
SIRWTKDARNTQDHVRSLCLLAWHNGKQEYEKFVSAIRSVPVGKALAIPN
YENLRRNWLELFH

3D structure

• PDB: 7w9s Crystal structure of a pre-chemistry viral RNA-dependent RNA polymerase suggests participation of two basic residues in catalysis.
• Chain: A
• Resolution: 2.53 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P20 E108 T114 S115 Y157 I176 A178 R188 H199 G211 C212 N213 G290 M291 S292 R416 L420	P20 E108 T114 S115 Y157 I176 A178 R188 H199 G211 C212 N213 G290 M291 S292 R416 L420
BS02	rna	A	S295 Y327 D330 K376 S401 N410 H414 S417	S295 Y327 D330 K376 S401 N410 H414 S417
BS03	CTP	A	D233 Y234 Y237 D238 S289 D329	D233 Y234 Y237 D238 S289 D329
BS04	ZN	A	H271 H273 C282	H271 H273 C282
BS05	MG	A	D233 D329 D330	D233 D329 D330
BS06	MG	A	D233 Y234 D329	D233 Y234 D329

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:7w9s, PDBe:7w9s, PDBj:7w9s
• PDBsum: 7w9s
• PubMed: 36477355
• UniProt: E5RPG3