Structure of PDB 7w5y Chain A

Receptor sequence
>7w5yA (length=300) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
TEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCA
VTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKS
GIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPA
STRIHSEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEM
ETNGTIDPEEAIRRAATILAEQLEAFVDLRDPILLRPVDDLELTVRSANC
LKAEAIHYIGDLVQRTEVELLKTPNLTEIKDVLASRGLSLGMRLENWPPA
3D structure
PDB7w5y Structural basis of three different transcription activation strategies adopted by a single regulator SoxS.
ChainA
Resolution4.2 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A V593 R594 N623 V245 R246 N275
BS02 dna A L619 P622 N623 L629 E631 L271 P274 N275 L276 E278
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046983 protein dimerization activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006879 intracellular iron ion homeostasis
GO:0009408 response to heat
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0036460 cellular response to cell envelope stress
GO:0042128 nitrate assimilation
GO:0044780 bacterial-type flagellum assembly
GO:0048870 cell motility
GO:0071973 bacterial-type flagellum-dependent cell motility
GO:0090605 submerged biofilm formation
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0000428 DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008023 transcription elongation factor complex
GO:0016020 membrane

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:7w5y, PDBe:7w5y, PDBj:7w5y
PDBsum7w5y
PubMed36243985
UniProtP0A7Z4|RPOA_ECOLI DNA-directed RNA polymerase subunit alpha (Gene Name=rpoA)

[Back to BioLiP]