Structure of PDB 7w4k Chain A

Receptor sequence

>7w4kA (length=431) Species: 9823 (Sus scrofa)

KKTSFGSLKDEDRIFTNLYGRHDWRLKGAQSRGDWYKTKEILLKGPDWIL
GEVKTSGLRGRGGAGFPTGLKWSFMNKPSDGRPKYLVVNADEGEPGTCKD
REIIRHDPHKLVEGCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAY
EAGLIGKNACGSGYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKP
PFPADVGVFGCPTTVANVETVAVSPTICRRGGAWFASFGRERNSGTKLFN
ISGHVNHPCTVEEEMSVPLKELIEKHAGGVIGGWDNLLAVIPGGSSTPLI
PKSVCETVLMDFDALVQAQTGLGTAAVIVMDRSTDIVKAIARLIEFYKHE
SCGQCTPCREGVDWMNKVMARFVKGDARPAEIDSLWEISKQIEGHTICAL
GDGAAWPVQGLIRHFRPELEERMQQFALQHQ

3D structure

• PDB: 7w4k The coupling mechanism of mammalian mitochondrial complex I.
• Chain: A
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	I205 P223 C379 G380 Q381 C382 C385 T423 C425 L427	I178 P196 C352 G353 Q354 C355 C358 T396 C398 L400
BS02	FMN	A	G87 R88 G89 N116 D118 E119 Y204 G207 E208 E209 A243 N244	G60 R61 G62 N89 D91 E92 Y177 G180 E181 E182 A216 N217
BS03	NAI	A	G89 A91 F93 F101 E121 Y204 E209 F229	G62 A64 F66 F74 E94 Y177 E182 F202

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0046872 metal ion binding
• GO:0051287 NAD binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006120 mitochondrial electron transport, NADH to ubiquinone
• GO:0042775 mitochondrial ATP synthesis coupled electron transport
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005829 cytosol
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:7w4k, PDBe:7w4k, PDBj:7w4k
• PDBsum: 7w4k
• PubMed: 35145322
• UniProt: A0A4X1SZP7