Structure of PDB 7w28 Chain A

Receptor sequence

>7w28A (length=478) Species: 9606 (Homo sapiens)

SPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRTLVEKIRKKQKGLSVT
FDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELF
LWVPRKLLMTVESAKNSVLGPLYSQDRILQAMGNIALAFHLLCERASPNS
FWQPYIQTLPSEYDTPLYFEEDEVRYLQSTQAIHDVFSQYKNTARQYAYF
YKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQIPTEDGSRVTLAL
IPLWDMCNHTNGLITTGYNLEDDRCECVALQDFRAGEQIYIFYGTRSNAE
FVIHSGFFFDNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFAL
HFTEPPISAQLLAFLRVFCMTEEELKEHLLGDSAIDRIFTLGNSEFPVSW
DNEVKLWTFLEDRASLLLKTYKTTIEEDKSVLKNHDLSVRAKMAIKLRLG
EKEILEKAVKSAAVNREYYRQQMEEKAP

3D structure

• PDB: 7w28 Histidine methyltransferase SETD3 methylates structurally diverse histidine mimics in actin.
• Chain: A
• Resolution: 1.79 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.85: protein-histidine N-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	M151 N153 N211 R214 Q215 Q254 N255 P258 W273 D274 I283 T285 G286 Y287 Y312 R315	M132 N134 N192 R195 Q196 Q235 N236 P239 W254 D255 I264 T266 G267 Y268 Y293 R296
BS02	SAH	A	R74 E103 F105 R253 D274 M275 N277 H278 Y312 S324 F326	R55 E84 F86 R234 D255 M256 N258 H259 Y293 S305 F307

Gene Ontology

Molecular Function

• GO:0018064 protein-L-histidine N-tele-methyltransferase activity

Biological Process

• GO:0030047 actin modification

External links

• PDB: RCSB:7w28, PDBe:7w28, PDBj:7w28
• PDBsum: 7w28
• PubMed: 35481649
• UniProt: Q86TU7|SETD3_HUMAN Actin-histidine N-methyltransferase (Gene Name=SETD3)