Structure of PDB 7vfs Chain A

Receptor sequence

>7vfsA (length=1266) Species: 9606 (Homo sapiens)

NVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLD
DTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILAT
AGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLF
FAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDT
ECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTW
NWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQI
ERELNGYLEWIFKAEEVMLAEEDRNSYFRRKEKMFRFFIRRMVKAQSFYW
VVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGL
GPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIF
KVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFN
FQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSS
FYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQ
KAKEVAARSVWEQRASQLRLQNLRASRRFCHYIVTMRYFEVVILVVIALS
SIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGA
YFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIK
RLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTD
ESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVST
GEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVA
LIIITFQEQKNERACIDFAISAPQNRQSFQYKTWTFVVSPPFEYFIMAMI
ALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYF
RDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQ
GYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTS
INRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGS
DFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIR
VWANDMFEMLKHMSVHFTSTLMALIRTALEITKQHQCDAELRKEISVVWA
NLPQKTLDLLVPPHKP

3D structure

• PDB: 7vfs Closed-state inactivation and pore-blocker modulation mechanisms of human Ca V 2.2.
• Chain: A
• Resolution: 2.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	Y01	A	T496 L497 A500 M501 N1350 V1351	T360 L361 A364 M365 N837 V838
BS02	Y01	A	N299 L301 F1492	N218 L220 F961
BS03	Y01	A	I1165 A1169 R1179 F1611	I652 A656 R666 F1080
BS04	PT5	A	S544 S545 F546 N547 L580 L583 R584 W592 L595 R596 V599 F1292 F1406	S408 S409 F410 N411 L444 L447 R448 W456 L459 R460 V463 F779 F893
BS05	CA	A	E314 E663 E1365	E233 E527 E852

Gene Ontology

Molecular Function

• GO:0001540 amyloid-beta binding
• GO:0005216 monoatomic ion channel activity
• GO:0005245 voltage-gated calcium channel activity
• GO:0005262 calcium channel activity
• GO:0005509 calcium ion binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008331 high voltage-gated calcium channel activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0006816 calcium ion transport
• GO:0007268 chemical synaptic transmission
• GO:0050804 modulation of chemical synaptic transmission
• GO:0055085 transmembrane transport
• GO:0070588 calcium ion transmembrane transport
• GO:0098703 calcium ion import across plasma membrane
• GO:1904645 response to amyloid-beta

Cellular Component

• GO:0005886 plasma membrane
• GO:0005891 voltage-gated calcium channel complex
• GO:0016020 membrane
• GO:0034702 monoatomic ion channel complex
• GO:0043025 neuronal cell body
• GO:0045202 synapse

External links

• PDB: RCSB:7vfs, PDBe:7vfs, PDBj:7vfs
• PDBsum: 7vfs
• PubMed: 34731621
• UniProt: Q00975|CAC1B_HUMAN Voltage-dependent N-type calcium channel subunit alpha-1B (Gene Name=CACNA1B)