Structure of PDB 7vbc Chain A

Receptor sequence

>7vbcA (length=1477) Species: 9606 (Homo sapiens)

NMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNPSANGLYDLAL
GPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSCL
NCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFSASEIREELE
QYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGR
SVVRKEHNSKLTITFRGYLTPTSAREHLSALWKNEGFFLNYLFSRFNPSV
FFLDFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMA
QEQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLF
RKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQ
ELRQAVINGPNVHPGASMVINEDGSRTALSAVDMTQREAVAKQLLTQGTK
IVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLRLHYANCKAYN
ADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMV
SGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPSILKPFPLWTGKQ
VVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQ
VIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGKVLTCLARLFT
AYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEA
ASYDEVRGKWQDAHLGKDQRDFNMIDLKFKEEVNHYSNEINKACMPFGLH
RQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLP
CFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSG
YLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQ
FPFLASNYEVIMKSQHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLLRR
GAFLSYSQKIQEAVKALKLESENRNGRSPGTQEMLRMWYELDEESRRKYQ
KKAAACPDPSLSVWRPDIYFASVSETFETKVDDYSQEWAAQTEKSYEKSE
LSLDRLRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMMNVTLGIPRL
REILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQKID
VQEQVYQLRFQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKKKNN
KASEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDMSS
LVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYA
EVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLS
LVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDE
LRSPSACLVVGKVVRGGTGLFELKQPL

3D structure

• PDB: 7vbc Structure of the human RNA polymerase I elongation complex.
• Chain: A
• Resolution: 3.01 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R552 G591	R466 G505
BS02	dna	A	L315 G316 R418 K423 K424 R429 Q553 R1659 T1676	L269 G270 R340 K345 K346 R351 Q467 R1417 T1434
BS03	dna	A	R101 N1258 R1663	R96 N1160 R1421
BS04	ZN	A	C64 H77	C59 H72
BS05	ZN	A	C104 C205	C99 C193

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0001054 RNA polymerase I activity
• GO:0003677 DNA binding
• GO:0003682 chromatin binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding
• GO:0071667 DNA/RNA hybrid binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006360 transcription by RNA polymerase I
• GO:1904750 negative regulation of protein localization to nucleolus

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005694 chromosome
• GO:0005730 nucleolus
• GO:0005736 RNA polymerase I complex

External links

• PDB: RCSB:7vbc, PDBe:7vbc, PDBj:7vbc
• PDBsum: 7vbc
• PubMed: 34671025
• UniProt: O95602|RPA1_HUMAN DNA-directed RNA polymerase I subunit RPA1 (Gene Name=POLR1A)