Structure of PDB 7v94 Chain A

Receptor sequence

>7v94A (length=1076) Species: 115547 (uncultured archaeon)

SIPLHAFRNSGADARKWKGRIALLAKRGKETMRTLQFPLEMSEPEAAAIN
TTPFAVAYNAIEGTGKGTLFDYWAKLHLAGFRFFPSGGAATIFRQQAVFE
DASWNAAFCQQSGKDWPWLVPSKLYERFTKAPREVASKKSIEFTQENVAN
ESHVSLVGASITDKTPEDQKEFFLKMAGALAEKFDSWKSANEDRIVAMKV
IDEFLKSEGLHLPSLENIAVKCSVETKPDNATVAWHDAPMSGVQNLAIGV
FATCASRIDNIYDLNGGKLSKLIQESATTPNVTALSWLFGKGLEYFRTTD
IDTIMQDFNIPASAKESIKPLVESAQAIPTMTVLGKKNYAPFRPNFGGKI
DSWIANYASRLMLLNDILEQIEPGFELPQALLDNETLMSGIDMTGDELKE
LIEAVYAWVDAAKQGLATLLGRGGNVDDAVQTFEQFSAMMDTLNGTLNTI
SARYVRAVEMAGKDEARLEKLIECKFDIPKWCKSVPKLVGISGGLPKVEE
EIKVMNAAFKDVRARMFVRFEEIAAYVASKGAGMDVYDALEKRELEQIKA
HIQAYRAVLHRIGRAVQNCSEKTKQLFSSKVIEMGVFKNPSHLNNFIFNQ
KGAIYRSPFDRSRHAPYQLHADKLLKNDWLELLAEISATLMASESTEQME
DALRLERTRLQLQLSGLPDWEYPASLAKPDIEVEIQTALKMQLAKDTVTS
DVLQRAFNLYSSVLSGLTFKLLRRSFSLKMRFSVADTTQLIYVPKVCDWA
IPKQYLQAEGEIGIAARVVTESSPAKMVTEVEMKEPKALGHFMQQAPHDW
YFDASLGGTQVAGRIVEKGKEVGKERKLVGYRMRGNSAYKTVLDKSLVGN
TELSQCSMIIEIPYTQTVDADFRAQVQAGLPKVSINLPVKETITAMLFDR
FVAIDLGERGLGYAVFDAKTLELQESGHRPIKAITNLLNRTHHYEQRPNQ
RQKFQAKFNVNLSELRENTVGDVCHQINRICAYYNAFPVLEYMVPDRLDK
QLKSVYESVTNRYIWSSTDAHKSARVQFWLGGETWEHPYLKSAKDKKPLV
LSPGRGAHADENAAVNIGGKFIADIE

3D structure

• PDB: 7v94 Structure of the type V-C CRISPR-Cas effector enzyme.
• Chain: A
• Resolution: 2.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	H9 A10 F11 R12 S14 G15 A16 R19 K20 R37 T38 R368 S397 R461 K495 V497 G498 S500 R578 R581 N585 F626 Q721 R722 N725 K746 R748 K762 W766 P769 Q771 Y772 A775 I779 Q812 P814 H815 R849 R851 G852 N853 S854 A855 Y856 K857 T858 N903 P953 N959 N962 R963 N972 R974 Q975 N982 N991 G994 H998 Q999 R1002 Q1050	H5 A6 F7 R8 S10 G11 A12 R15 K16 R33 T34 R360 S389 R453 K487 V489 G490 S492 R561 R564 N568 F609 Q704 R705 N708 K729 R731 K745 W749 P752 Q754 Y755 A758 I762 Q795 P797 H798 R832 R834 G835 N836 S837 A838 Y839 K840 T841 N886 P930 N936 N939 R940 N949 R951 Q952 N959 N968 G971 H975 Q976 R979 Q1027
BS02	dna	A	M36 R137 E138 A140 Q282 N289 G356 D359 S360 A363 N364 G453 N456 T457 A460 F626 D627 R628 E701 Q703 S729 F736 K835 Q872 Q975 R989 S1027 T1041 A1043 H1044	M32 R133 E134 A136 Q274 N281 G348 D351 S352 A355 N356 G445 N448 T449 A452 F609 D610 R611 E684 Q686 S712 F719 K818 Q855 Q952 R966 S1004 T1018 A1020 H1021
BS03	dna	A	P136 R137 V139 K147 N238 N289 T291 S294 K299 K345 N346	P132 R133 V135 K139 N230 N281 T283 S286 K291 K337 N338

External links

• PDB: RCSB:7v94, PDBe:7v94, PDBj:7v94
• PDBsum: 7v94
• PubMed: 35366394