Structure of PDB 7uy6 Chain A

Receptor sequence

>7uy6A (length=1012) Species: 5911 (Tetrahymena thermophila)

KQMLTRKEDLLTVLKQISALKYVSNLYEFLLATEKIVQTSELDTQFQEFL
TTTIIASEQNLVENYKQKYNQPNFSQLTIKQVIDDSIILLGNKQNYVQQI
GTTTIGFYVEYENINLSRQTLYSSNFRNLLNIFGEEDFKYFLIDFLVFTK
VEQNGYLQVAGVCLNQYFSENQYIYPEIQRSQIFYCNHMGREPGVFKSSF
FNYSEPQTIIKKTLLKEYQSKNFSCQEERDLFLEFTEKIVQNFHNINFNY
LLKKFCKLPENYQSLKSQVKQIVQSENKANQQSCENLFNSLYDTEISYKQ
ITNFLRQIIQNCVPNQLLGKKNFKVFLEKLYEFVQMKRFENQKVLDYICF
MDVFDVEWFVDLKNQKFTQKRKYISDKRKILGDLIVFIINKIVIPVLRYN
FYITEKHKEGSQIFYYRKPIWKLVSKLTIVKLEEENLEKVEEKLIPEDSF
QKYPQGKLRIIPKKGSFRPIMTFLRKDKQKNIKLNLNQILMDSQLVFRNL
KDMLGQKIGYSVFDNKQISEKFAQFIEKWKNKGRPQLYYVTLDIKKCYDS
IDQMKLLNFFNQSDLIQDTYFINKYLLFQRNKRPLLQIMDNINFPYYFNL
KERQIAYSLYDDDDQILQKGFKEIQSDDRPFIVINQDKPRCITKDIIHNH
LKHISQYNVISFNKVKFRQKRGIPQGLNISGVLCSFYFGKLEEEYTQFLK
NAEQVNGSINLLMRLTDDYLFISDSQQNALNLIVQLQNCANNNGFMFNDQ
KITTNFQFPQEDYNLEHFKISVQNECQWIGKSIDMNTLEIKSIQKQTQQE
INQTINVAISIKNLKSQLKNKLRSLFLNQLIDYFNPNINSFEGLCRQLYH
HSKATVMKFYPFMTKLFQIDLKKSKQYSVQYGKENTNENFLKDILYYTVE
DVCKILCYLQFEDEINSNIKEIFKNLYSWIMWDIIVSYLKKKKQFKGYLN
KLLQKIRKSRFFYLKEGCKSLQLILSQQKYQLNKKELEAIEFIDLNNLIQ
DIKTLIPKISAK

3D structure

• PDB: 7uy6 Structure of Tetrahymena telomerase-bound CST with polymerase alpha-primase.
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.49: RNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Y231 H234 M235 R237 F242 K243 S244 N324 Y325 K328 K329 L333 Y337 K341 K374 Q382 L420 D421 C424 V428 Q444 K445 R446 K447 I449 S450 R453 R473 R492 R534 I545 L549 R550 K551 D552 K553 K576 K657 R658 P693 Y694 N697 G774 L775 N776 I909 K910 K913 S914 K917 R921 S922 L925 I967 K971 S972 K973 V977 K990 Y994 Y995 K1038 K1040 K1041 Q1052 S1057 F1059 F1060 Y1061 K1063 K1101	Y185 H188 M189 R191 F196 K197 S198 N249 Y250 K253 K254 L258 Y262 K266 K299 Q307 L345 D346 C349 V353 Q369 K370 R371 K372 I374 S375 R378 R398 R417 R459 I470 L474 R475 K476 D477 K478 K501 K582 R583 P595 Y596 N599 G676 L677 N678 I811 K812 K815 S816 K819 R823 S824 L827 I869 K873 S874 K875 V879 K892 Y896 Y897 K940 K942 K943 Q954 S959 F961 F962 Y963 K965 K1003
BS02	dna	A	F414 L559 I877 T902 N904 Q927 K956	F339 L484 I779 T804 N806 Q829 K858

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003720 telomerase activity
• GO:0003723 RNA binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0042162 telomeric DNA binding
• GO:0046872 metal ion binding
• GO:0070034 telomerase RNA binding

Biological Process

• GO:0007004 telomere maintenance via telomerase

Cellular Component

• GO:0000333 telomerase catalytic core complex
• GO:0000781 chromosome, telomeric region
• GO:0005634 nucleus
• GO:0005694 chromosome
• GO:0005697 telomerase holoenzyme complex
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:7uy6, PDBe:7uy6, PDBj:7uy6
• PDBsum: 7uy6
• PubMed: 35831498
• UniProt: O77448|TERT_TETTS Telomerase reverse transcriptase (Gene Name=TERT)