Structure of PDB 7un7 Chain A

Receptor sequence

>7un7A (length=319) Species: 9606 (Homo sapiens)

HNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACS
IPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQM
WYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQK
AAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSL
RQEGFLTDDLVKGETKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYF
TGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGAKVGPGRVLPTPTE
KDVFRLLGLPYREPAERDW

3D structure

• PDB: 7un7 Pol lambda promotes microhomology-mediated end-joining.
• Chain: A
• Resolution: 2.04 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 R514 R517 K521 L527 H541	W23 R258 R261 K265 L271 H285
BS02	dna	A	V462 K463 G464 T471 K472 H530	V211 K212 G213 T215 K216 H274
BS03	dna	A	W342 G343 A344 G345 K347 T348 R488 D490 Y505	W91 G92 A93 G94 K96 T97 R232 D234 Y249
BS04	dna	A	W274 R275 G278 Y279 P303 G304 G306 R308 M309 K312	W23 R24 G27 Y28 P52 G53 G55 R57 M58 K61
BS05	TTP	A	R386 S417 R420 G426 D427 D429 Y505 T507 G508 A510 N513	R135 S166 R169 G175 D176 D178 Y249 T251 G252 A254 N257
BS06	CA	A	D427 D429	D176 D178
BS07	CA	A	D427 D429 D490	D176 D178 D234

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:7un7, PDBe:7un7, PDBj:7un7
• PDBsum: 7un7
• PubMed: 36536104
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)