Structure of PDB 7ule Chain A

Receptor sequence

>7uleA (length=248) Species: 224325 (Archaeoglobus fulgidus DSM 4304) [Search protein sequence]

RVEVFPVEGLPLIKEGDDLAELISSRVRFEDGDVLVVCSTVISKAEGRIR
RLEEFNPSERAKEIAARIGKPAEFVQAVLEESEEVLLDFPFLLVKAKFGN
VCVNAGIDASNVEEGSLLLPPLDPDGSAEKLRRRILELTGKRVGVIITDT
NGRCFRRGVVGFAIGISGVKAMKDWIGRKDLYGRELEVTVECVADEIAAF
ANLLMGEGGDGIPAVVVRGLNVAGEGSMEEIYRSEEEDVIRRCLKRCL

3D structure

PDB

7ule A Universal Mechanism for Poly-glutamylation

Chain

Resolution

1.7 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

6.3.2.31: coenzyme F420-0:L-glutamate ligase.
6.3.2.34: coenzyme F420-1:gamma-L-glutamate ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	GDP	A	L11 P12 L13 I14 C39 S40 T41 V42 K45 N112 D150 T151 M206 G207 G209 G212 I213 P214	L10 P11 L12 I13 C38 S39 T40 V41 K44 N111 D149 T150 M205 G206 G208 G211 I212 P213
BS02	F4I	A	F92 V104 D109 A110 S111 T151 N152 G153 R154	F91 V103 D108 A109 S110 T150 N151 G152 R153
BS03	MN	A	T151 E208	T150 E207
BS04	MN	A	D109 D150	D108 D149

Gene Ontology

	Molecular Function
GO:0005525	GTP binding
GO:0016874	ligase activity
GO:0043773	coenzyme F420-0 gamma-glutamyl ligase activity
GO:0046872	metal ion binding
GO:0052618	coenzyme F420-0:L-glutamate ligase activity
GO:0052619	coenzyme F420-1:gamma-L-glutamate ligase activity

	Biological Process
GO:0052645	F420-0 metabolic process

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Molecular Function

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Biological Process

External links

PDB	RCSB:7ule, PDBe:7ule, PDBj:7ule
PDBsum	7ule
PubMed
UniProt	O28028\|COFE_ARCFU Coenzyme F420:L-glutamate ligase (Gene Name=cofE)

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