Structure of PDB 7uaf Chain A

Receptor sequence

>7uafA (length=535) Species: 9606 (Homo sapiens)

NGLRDPNTRWTFPIPYILADNLGLNAKGAILYAFEMFRLKSCVDFKPYEG
ESSYIIFQQFDGCWSEVGDQHVGQNISIGQGCAYKAIIEHEILHALGFYH
EQSRTDRDDYVNIWWDQILSGYQHNFDTYDDSLITDLNTPYDYESLMHYQ
PFSFNKNASVPTITAKIPEFNSIIGQRLDFSAIDLERLNRMYNCTTTHTL
LDHCTFEKANICGMIQGTRDDTDWAHQDSAQAGEVDHTLLGQCTGAGYFM
QFSTSSGSAEEAALLESRILYPKRKQQCLQFFYKMTGSPSDRLVVWVRRD
DSTGNVRKLVKVQTFQGDDDHNWKIAHVVLKEEQKFRYLFQGTKGDPQNS
TGGIYLDDITLTETPCPTGVWTVRNFSQVLENTSKGDKLQSPRFYNSEGY
GFGVTLYPNSRESSGYLRLAFHVCSGENDAILEWPVENRQVIITILDQEP
DVRNRMSSSMVFTTSKSHTSPAINDTVIWDRPSRVGTYHTDCNCFRSIDL
GWSGFISHQMLKRRSFLKNDDLIIFVDFEDITHLS

3D structure

• PDB: 7uaf Helical ultrastructure of the metalloprotease meprin alpha in complex with a small molecule inhibitor.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 3.4.24.18: meprin A.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FUC	A	T351 G352 N414 T416	T286 G287 N349 T351
BS02	M6X	A	C128 C147 I152 H155 E156 L211 H213 Y214 Q241 R242	C63 C82 I87 H90 E91 L146 H148 Y149 Q176 R177
BS03	CA	A	G282 D285 T287 D288	G217 D220 T222 D223
BS04	ZN	A	H155 H159 H165	H90 H94 H100
BS05	CA	A	T270 E272 D301 T303 Y313 D422	T205 E207 D236 T238 Y248 D357

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:7uaf, PDBe:7uaf, PDBj:7uaf
• PDBsum: 7uaf
• PubMed: 36261433
• UniProt: Q16819|MEP1A_HUMAN Meprin A subunit alpha (Gene Name=MEP1A)