Structure of PDB 7u25 Chain A

Receptor sequence

>7u25A (length=582) Species: 3702 (Arabidopsis thaliana)

TFISRFAPDQPRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSSS
IRNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSGLADALLD
SVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRII
EEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPGYMSRMPKPPE
DSHLEQIVRLISESKKPVLYVGGGCLNSSDELGRFVELTGIPVASTLMGL
GSYPEDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRVTGKLEAF
ASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKL
DFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGV
GQHQMWAAQFYNYKKPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDI
DGDGSFIMNVQELATIRVENLPVKVLLLNNQHLGMVMQLEDRFYKANRAH
TFLGDPAQEDEIFPNMLLFAAACGIPAARVTKKADLREAIQTMLDTPGPY
LLDVICPHQEHVLPMIPSGGTFNDVITEGDGR

3D structure

• PDB: 7u25 Structural basis of resistance to herbicides that target acetohydroxyacid synthase.
• Chain: A
• Resolution: 3.19 Å

Enzymatic activity

• Enzyme Commision number: 2.2.1.6: acetolactate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	D538 N565 H567	D453 N480 H482
BS02	6QL	A	M351 R377 L574 S653	M266 R292 L489 S568
BS03	FAD	A	R246 G307 G308 G309 T331 L332 M333 L349 G371 V372 R373 D375 R377 V378 D395 I396 D414 V415 M490 G508	R161 G222 G223 G224 T246 L247 M248 L264 G286 V287 R288 D290 R292 V293 D310 I311 D329 V330 M405 G423
BS04	TZD	A	V485 G486 Q487 H488 G511 M513 D538 G539 S540 N565 H567 L568 G569 M570 V571	V400 G401 Q402 H403 G426 M428 D453 G454 S455 N480 H482 L483 G484 M485 V486

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0003984 acetolactate synthase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0009082 branched-chain amino acid biosynthetic process

External links

• PDB: RCSB:7u25, PDBe:7u25, PDBj:7u25
• PDBsum: 7u25
• PubMed: 35690625
• UniProt: P17597|ILVB_ARATH Acetolactate synthase, chloroplastic (Gene Name=ALS)